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Gamma carbonic anhydrase like complex interact with plant mitochondrial complex I

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Abstract

We report the identification by two hybrid screens of two novel similar proteins, called Arabidopsis thaliana gamma carbonic anhydrase like1 and 2 (AtγCAL1 and AtγCAL2), that interact specifically with putative Arabidopsis thaliana gamma Carbonic Anhydrase (AtγCA) proteins in plant mitochondria. The interaction region that was located in the N-terminal 150 amino acids of mature AtγCA and AtγCA like proteins represents a new interaction domain. In vitro experiments indicate that these proteins are imported into mitochondria and are associated with mitochondrial complex I as AtγCAs. All plant species analyzed contain both AtγCA and AtγCAL sequences indicating that these genes were conserved throughout plant evolution. Structural modeling of AtγCAL sequences show a deviation of functionally important active site residues with respect to γCAs but could form active interfaces in the interaction with AtγCAs. We postulate a CA complex tightly associated to plant mitochondrial complex.

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Authors and Affiliations

  1. Instituto de Investigaciones Biotecnológicas, IIB-INTECH (CONICET/UNSAM), C.C. 164, Chascomús, Argentina, 7130

    Mariano Perales, Alejandro Colaneri, Fernando Villarreal, Nahuel González-Schain, Diego Gómez-Casati & Eduardo Zabaleta

  2. Centro de Estudios de Investigaciones, Universidad Nacional de Quilmes, Roque Sáenz Peña 180, Bernal., Argentina, B1876BXD

    Gustavo Parisi, María Silvina Fornasari & Julián Echave

  3. Institut für Angewandte Genetik, Universität Hannover, Herrenhäuser Str. 2, Hannover, Germany, D-30419

    Hans-Peter Braun

  4. UMR 5097 R.E.G.E.R., CNRS and Université Victor Segalen Bordeaux 2, 146 rue Léo Saignat, Bordeaux, France, 33076

    Alejandro Araya

  5. Instituto de Investigaciones Biológicas, Universidad Nacional de Mar del Plata, Funes 3250, Mar del Plata, Argentina, 7600

    Eduardo Zabaleta

Authors
  1. Mariano Perales
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  2. Gustavo Parisi
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  3. María Silvina Fornasari
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  4. Alejandro Colaneri
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  5. Fernando Villarreal
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  6. Nahuel González-Schain
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  7. Julián Echave
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  8. Diego Gómez-Casati
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  9. Hans-Peter Braun
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  10. Alejandro Araya
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  11. Eduardo Zabaleta
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Correspondence to Eduardo Zabaleta.

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Perales, M., Parisi, G., Fornasari, M.S. et al. Gamma carbonic anhydrase like complex interact with plant mitochondrial complex I. Plant Mol Biol 56, 947–957 (2004). https://doi.org/10.1007/s11103-004-6324-z

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  • DOI: https://doi.org/10.1007/s11103-004-6324-z

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