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Functional conservation and maintenance of expression pattern of FIDDLEHEAD-like genes in Arabidopsis and Antirrhinum

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Abstract

In Arabidopsis, loss of function of the epidermis-specific FDH gene coding for a putative β-ketoacyl-CoA synthase results in ectopic organ fusions in mutants. Corresponding mutants are not available for Antirrhinum majus, however, organ fusions can be induced in both species by chloroacetamide inhibitors of β-ketoacyl-CoA synthases using a chemical genetics approach. We isolated the ortholog of FDH from Antirrhinum majus, the ANTIRRHINUM FIDDLEHEAD (AFI ) gene, and showed that AFI complements fdh when expressed in the epidermis under control of the FDH promoter. Like FDH, the AFI gene exhibits protodermis- and epidermis-specific expression, and its promoter directs the expression of reporter genes to the epidermis in transgenic Antirrhinum and Arabidopsis. We demonstrate down-regulation of the FDH promoter in the epidermis of the ovary septum, thereby supporting the assumption that FDH-like genes may directly facilitate the cell–cell interactions that need to occur during carpel fusion and pollen tube growth. Up-regulation of FDH in the stomium, on the other hand, provides evidence for its possible involvement in cell separation during anther dehiscence. Down-regulation of the FDH and AFI promoters in the septum is observed in transgenic Arabidopsis but not in Antirrhinum plants. This probably reflects differences in the ontogeny of the ovary septum between the two species. We also show that epidermis-specific FDH-like genes may not be able to efficiently elongate fatty acid chains when misexpressed in seeds.

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Authors and Affiliations

  1. Max-Planck-Institut für Züchtungsforschung, Carl-von-Linné-Weg 10, 50829, Köln, Germany

    Nadia Efremova, Sascha Bär, Iris Heidmann, Peter Huijser, Kirsten Wellesen, Zsuzsanna Schwarz-Sommer, Heinz Saedler & Alexander Yephremov

  2. Institut für Zelluläre and Molekulare Botanik, Universität Bonn, Kirschallee 1, D-53115, Bonn, Germany

    Lukas Schreiber

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  1. Nadia Efremova
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  9. Alexander Yephremov
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Correspondence to Alexander Yephremov.

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Efremova, N., Schreiber, L., Bär, S. et al. Functional conservation and maintenance of expression pattern of FIDDLEHEAD-like genes in Arabidopsis and Antirrhinum. Plant Mol Biol 56, 821–837 (2004). https://doi.org/10.1007/s11103-004-5576-y

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