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Gene cloning and characterization of a soybean (Glycine max L.) LEA protein, GmPM16

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Abstract

Late embryogenesis abundant (LEA) proteins, present in abundance in seeds during the late stages of development, are associated with desiccation tolerance. In the present work, we characterize a soybean LEA protein, GmPM16, with low molecular weight, high pI value, and an unusual amino acid residue distribution along the protein. The transcripts were detected in cotyledon mesophyll cells but not in the vascular system of mature or pod-dried soybean seeds. Circular dichroism (CD) analysis and Fourier transfer infrared (FTIR) spectroscopy indicated that the GmPM16 protein in solution was highly unordered, possessing only partial α-helical structures. However, the protein in sodium dodecyl sulfate (SDS) or trifluoroethanol (TFE) solution or in a dry state exhibited a conformation of abundant α-helical structures. As well, the GmPM16 protein interacts with sugar and forms tightly glassy matrixes in the dry state. The protein may play a role in reducing cellular damage in drying seeds by changing the protein conformation and forming tight cellular glasses.

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Authors and Affiliations

  1. Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan

    Ming-der Shih

  2. Department of Agronomy, National Taiwan University, Taipei, Taiwan

    Shu-chin Lin & Jaw-shu Hsieh

  3. Institute of Botany, Academia Sinica, Taipei, Taiwan

    Chi-hua Tsou, Teh-yuan Chow & Yue-ie C. Hsing

  4. Department of Life Sciences, National Taiwan University, Taipei, Taiwan

    Tsai-piao Lin

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  1. Ming-der Shih
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  2. Shu-chin Lin
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  3. Jaw-shu Hsieh
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  4. Chi-hua Tsou
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  5. Teh-yuan Chow
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  6. Tsai-piao Lin
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  7. Yue-ie C. Hsing
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Correspondence to Yue-ie C. Hsing.

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Shih, Md., Lin, Sc., Hsieh, Js. et al. Gene cloning and characterization of a soybean (Glycine max L.) LEA protein, GmPM16. Plant Mol Biol 56, 689–703 (2004). https://doi.org/10.1007/s11103-004-4680-3

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