Abstract
Cassava mosaic disease (CMD) is a viral disease of the important tropical staple crop cassava (Manihot esculenta) and preferred management involves use of host–plant resistance. The best available resistance is controlled by a single dominant gene. Serial analysis of gene expression (SAGE) was used to analyze the gene expression pattern in a bulk of 40 each of CMD resistant and susceptible genotypes drawn from a gene mapping progeny. Messenger RNA used for the SAGE analysis came from plants that were exposed to heavy disease pressure over a period of 2 years in the field. A total of 12,786 tags were studied, divided into 5733 and 7053 tags from the resistant and susceptible genotypes, respectively. Tag annotation was by PCR amplification using the tag sequence as sense primer and 4000 cassava ESTs generated from the bulk of CMD resistant genotypes. Annotation of more than 30 differentially expressed tags revealed several genes expressed during systemic acquired resistance (SAR) in plants and other genes involved in cell-to-cell and cytoplasm-to-nucleus virus trafficking. Differential expression of the most abundantly expressed tag, corresponding to a beta-tubulin gene, was confirmed by Northern Analysis. RFLP analysis of the tags in the parents and bulks of the CMD mapping progeny revealed only one tag, a WRKY transcription factor, associated with the region bearing the dominant CMD gene.
Similar content being viewed by others
References
A. Akano E. Barrera C. Mba A.G.O. Dixon M. Fregene (2002) ArticleTitleGenetic mapping of a dominant gene conferring resistance to the cassava mosaic disease Theor. and Appl. Genet. 105 521–525 Occurrence Handle10.1007/s00122-002-0891-7
S.F. Altschul T.L. Madden A.A. Shaffer J. Zhang Z. Zhang W. Miller D.J. Lipman (1997) ArticleTitleGapped BLAST and PSI-BLAST: a new generation of protein database search programs Nucleic Acids Res. 25 3389–3402 Occurrence Handle10.1093/nar/25.17.3389 Occurrence Handle9254694
J.L. Blackwell M.A. Brinton (1997) ArticleTitleTranslation elongation factor-1 alpha interacts with the 3′stem-loop region of West Nile virus genomic RNA Virology 71 IssueID9 6433–6444
V. Boyko J. Ferralli J. Ashby P. Schellenbaum M. Heinlein (2000) ArticleTitleFunction of microtubules in intercellular transport of plant virus RNA Cell Biol 2 IssueID11 826–832
CIAT.2001 Assessing and utilizing agrobiodiversity through biotechnology Annual Report 2001. Project SB-02. Centro Internacional de Agricultura Tropical Cali, Colombia
A. Cimarelli J. Luban (1999) ArticleTitleTranslation elongation factor 1-alpha interacts specifically with the human immunodeficiency virus type 1 Gag polyprotein J Virol. 73 IssueID7 5388–5401 Occurrence Handle10364286
A.P. de Matos Z.G. Carvalho (1993) ArticleTitleAfrican swine fever virus interaction with microtubules Biol. Cell 78 IssueID3 229–234 Occurrence Handle10.1016/0248-4900(93)90134-Z Occurrence Handle8241964
C. Fauquet D. Fargette (1990) ArticleTitleAfrican cassava mosaic virus Etiology, epidemiology and control. Plant Disease 74 IssueID6 404–411
R.S.S. Fraser (1986) ArticleTitleGenes for resistance to plant viruses CRC Crit. Rev. Plant Sci. 3 257–294
M. Fregene F. Angel R. Gomez F. Rodriguez P. Chavarriaga W. Roca J. Tohme M. Bonierbale (1997) ArticleTitleA molecular genetic map of cassava (Manihot esculenta Crantz) Theor. Appl. Genet. 95 431–441 Occurrence Handle10.1007/s001220050580
M. Fregene A. Bernal M. Duque A. Dixon J. Tohme (2000) ArticleTitleAFLP analysis of African cassava (Manihot esculenta Crantz) germplasm resistant to the cassava mosaic disease (CMD) Theor. Appl. Genet. 100 678–685
S.K Hahn E.R. Terry K. Leuschner (1980) ArticleTitleBreeding cassava for resistance to cassava mosaic disease Euphytica 29 673–683
D.L.Jennings1976 Breeding for Resistance to African Cassava Mosaic African cassava mosaic Report of an interdisciplinary Workshop held at Muguga, Kenya. IDRC-071e, pp. 39–44
N.T.Keen,A.Bent,B. Staskawicz 1993 In: I Chet (Ed.) Plant Disease Resistance Genes: Interactions with Pathogens and Their Improved Utilization to Control Plant Diseases Wiley/Liss New York, pp. 65–88
B.A. Kohm M.G. Goulden J.E. Gilbert T.A. Kavanagh D.C. Baulcombe (1993) ArticleTitleA potato virus X resistance gene mediates an induced, nonspecific resistance in protoplasts Plant Cell 5 913–920 Occurrence Handle12271089
H. Matsumura N. Shizuko R. Terauchi (1999) ArticleTitleTranscript profiling in rice (Oryza sativa L.) seedling using serial analysis of gene expression Plant J. 20 IssueID6 719–726 Occurrence Handle10652144
B.G. McLean J. Zupan P.C. Zambryski (1995) ArticleTitleTobacco mosaic virus movement protein associates with the cytoskeleton in tobacco cells Plant Cell 7 IssueID12 2101–14 Occurrence Handle8718621
Ogbe, F.O., Atiri, G.I., Dixon, A.G.O. and Thottappilly G. 2003. Symptom severity of cassava mosaic disease in relation to concentration of African cassava mosaic virus in different cassava genotypes Plant Pathology 52: 84–91.
K. Polyak Y. Xia J.L. Zweier K.W. Kinzler B. Vogelstein (1997) ArticleTitleA model for P53-induced Apoptosis Nature 389 300–305 Occurrence Handle9305847
P.G. Rajendran,S.G.Nair,A.Easwari,K.Vasudevan,M.T.Sreekumari,1993Cassava breeding, agronomy research and technology transfer in AsiaIn: R.H. Howeler (Ed.) Proceeding of the Fourth Regional Workshop Held in TrivandrumKerala, India. pp. 84–96
J. Sambrook E.F. Fritsch T. Maniatis (1989) Molecular Cloning, a Laboratory Manual Cold Spring Harbor Laboratory Press New York
H.H. Storey R.F.W. Nichols (1938) ArticleTitleStudies on the mosaic disease of cassava Ann. Appl. Biol. 25 IssueID4 709–806
M.M. Swanson B.D. Harrison (1994) ArticleTitleProperties, relationships and distribution of cassava mosaic geminiviruses Trop. Sci. 34 15–25
Truant, R. and Cullen, B.R. 1999. The arginine-rich domains present in human immunodeficiency virus type 1 Tat and Rev function as direct importin.
V.E. Velculescu L. Zhang W. Zhou B. Vogelstein M.A. Basrai D.E. Bassett P. Hieter J. Vogelstein K.W. Kinzler (1997) ArticleTitleCharacterization of yeast transcriptomes Cell 88 243–251 Occurrence Handle9008165
S. Whitham S.P. Dinesh-Kumar D. Choi R. Hehl C. Corr B.J. Baker (1994) ArticleTitleThe product of the tobacco mosaic virus resistance gene N: similarity to toll and the interleukin-1 receptor Cell 78 1101–1115 Occurrence Handle7923359
L. Zhang W. Zhou V.E. Velculescu S.E. Kern R. H. Hruban S. R. Hamilton B. Vogelstein K.W. Kinzler (1997) ArticleTitleGene expression profiles in normal and cancer cells Science 276 1268–1272 Occurrence Handle9157888
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Fregene, M., Matsumura, H., Akano, A. et al. Serial analysis of gene expression (SAGE) of host–plant resistance to the cassava mosaic disease (CMD). Plant Mol Biol 56, 563–571 (2004). https://doi.org/10.1007/s11103-004-3477-8
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11103-004-3477-8