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Improvement of grapevine physiology and yield under summer stress by kaolin-foliar application: water relations, photosynthesis and oxidative damage

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Photosynthetica

Abstract

Knowledge about short-term climate change adaptation strategies for Mediterranean vineyards is needed in order to improve grapevine physiology and yield-quality attributes. We investigated effects of kaolin-particle film suspension on water relations, photosynthesis and oxidative stress of field-grown grapevines in the Douro region (northern Portugal) in 2012 and 2013. Kaolin suspension decreased leaf temperature by 18% and increased leaf water potential (up to 40.7% in 2013). Maximum photochemical quantum efficiency of PSII was higher and the minimal chlorophyll fluorescence was lower in the plants sprayed by kaolin. Two months after application, net photosynthesis and stomatal conductance at midday increased by 58.7 and 28.4%, respectively, in treated plants. In the same period, kaolin treatment increased photochemical reflectance, photosynthetic pigments, soluble proteins, soluble sugars, and starch concentrations, while decreased total phenols and thiobarbituric acid-reactive substances. Kaolin application can be an operational tool to alleviate summer stresses, which ameliorates grapevine physiology and consequently leads to a higher yield.

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Abbreviations

Chl:

chlorophyll

Car:

carotenoid

C i/C a :

internal CO2 concentration/ambient CO2 ratio

E :

transpiration rate

ETR:

photosynthetic electron transport rate

F0 :

minimal chlorophyll fluorescence

Fv :

variable chlorophyll fluorescence

Fm :

maximal chlorophyll fluorescence

Fv/Fm :

maximum photochemical quantum efficiency of PSII

Fv’/Fm’:

capture efficiency of excitation energy by open PSII reaction centres

g s :

stomatal conductance

P N :

net CO2 assimilation rate

qN :

nonphotochemical quenching

qP :

photochemical quenching

RIred edge :

chlorophyll reflectance index

SP:

soluble proteins

SS:

soluble sugars

St:

starch

TBARS:

thiobarbituric acid-reactive substances

TP:

total phenols

ΦPSII :

actual PSII efficiency

Ψmd :

midday leaf water potential

Ψpd :

predawn leaf water potential

Ψpd :

WUEi-PN/gs, intrinsic water-use efficiency

References

  • Ashraf M., Harris P.J.C.: Potential biochemical indicators of salinity tolerance in plants. — Plant Sci. 166: 3–16, 2004.

    Article  CAS  Google Scholar 

  • Bacelar E.A., Santos D.L., Moutinho-Pereira J.M. et al.: Immediate responses and adaptative strategies of three olive cultivars under contrasting water availability regimes: Changes on structure and chemical composition of foliage and oxidative damage. — Plant Sci. 170: 596–605, 2006.

    Article  CAS  Google Scholar 

  • Baker N.R.: Chlorophyll fluorescence: a probe of photosynthesis in vivo. — Annu. Rev. Plant Biol. 59: 89–113, 2008.

    Article  CAS  PubMed  Google Scholar 

  • Bilger W., Schreiber U.: Energy-dependent quenching of darklevel chlorophyll fluorescence in intact leaves. — Photosynth. Res. 10: 303–308, 1986.

    Article  CAS  PubMed  Google Scholar 

  • Bradford M.M.: A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. — Anal. Biochem. 72: 248–254, 1976.

    Article  CAS  PubMed  Google Scholar 

  • Centritto M., Wahbi S., Serraj R., Chaves M.M.: Effects of partial root–zone (PRD) on adult olive tree (Olea europaea) in field conditions under arid climate. II. Photosynthetic responses. — Agr. Ecosyst. Environ. 106: 303–311, 2005.

    Article  Google Scholar 

  • Chaves M.M., Santos T., Souza C.R. et al.: Deficit irrigation in grapevine improves water-use efficiency while controlling vigour and production quality. — Ann. Appl. Biol. 150: 237–252, 2007.

    Article  Google Scholar 

  • Costa H., Gallego, S.M., Tomaro, M.L.: Effect of UV-B radiation on antioxidant defense system in sunflower cotyledons. — Plant Sci. 162: 939–945, 2002.

    Article  CAS  Google Scholar 

  • DaMatta F.M., Loos R.A., Silva E.A., Loureiro M.E.: Limitations to photosynthesis in Coffea canephora as a result of nitrogen and water availability. — J. Plant Physiol. 159: 975–981, 2002.

    Article  CAS  Google Scholar 

  • Dinis L.-T., Correia C.M., Ferreira H.F., Gonçalves B. et al.: Physiological and biochemical responses of Semillon and Muscat Blanc à Petits Grains winegrapes grown under Mediterranean climate. — Sci. Hortic.-Amsterdam 175: 128–138, 2014.

    Article  CAS  Google Scholar 

  • Dinis L.-T., Ferreira H., Pinto G. et al.: Kaolin-based, foliar reflective film protects photosystem II structure and function in grapevine leaves exposed to heat and high solar radiation. — Photosynthetica 54: 47–55, 2016a.

    Article  CAS  Google Scholar 

  • Dinis L.-T., Bernardo S., Conde A. et al.: Kaolin exogenous application boosts antioxidant capacity and phenolic content in berries and leaves of grapevine under summer stress. — J. Plant Physiol. 191: 45–53, 2016b.

    Article  CAS  PubMed  Google Scholar 

  • dos Santos T.P., Lopes C.M., Rodrigues M.L. et al.: Effects of deficit irrigation strategies on cluster microclimate for improving fruit composition of Moscatel field-grown grapevines. — Sci. Hortic.-Amsterdam 112: 321–330, 2007.

    Article  Google Scholar 

  • Epron D., Dreyer E. Bréda N.: Photosynthesis of oak trees (Quercus petraea (Matt) Liebl.) during drought stress under field conditions: diurnal course of net CO2 assimilation and photochemical efficiency of photosystem II. — Plant Cell Environ. 15: 809–820, 1992.

    Article  CAS  Google Scholar 

  • FAO: World Reference Base for Soil Resources 2014, update 2015. International Soil Classification System for Naming Soils and Creating Legends for Soil Maps. World Soil Resources Reports No. 106. FAO, Rome 2015.

  • Ferrandino A., Lovisolo C.: Abiotic stress effects on grapevine (Vitis vinifera L.): Focus on abscisic acid-mediated consequences on secondary metabolism and berry quality. — Environ. Exp. Bot. 103: 138–147, 2014.

    Article  CAS  Google Scholar 

  • Fraga H., Malheiro A.C., Moutinho-Pereira J., Santos J.A.: Future scenarios for viticultural zoning in Europe: ensemble projections and uncertainties. — Int. J. Biometeorol. 57: 909–925, 2013.

    Article  CAS  PubMed  Google Scholar 

  • Fraga H., Malheiro A.C., Moutinho-Pereira J. et al.: Very high resolution bioclimatic zoning of Portuguese wine regions: present and future scenarios. — Reg. Environ. Change 14: 295–306, 2014a.

    Article  Google Scholar 

  • Fraga H., Malheiro A.C., Moutinho-Pereira J., Santos J.A.: Climate factors driving wine production in the Portuguese Minho region. — Agr. Forest Meteorol. 185: 26–36, 2014b.

    Article  Google Scholar 

  • Fraga H., de Cortázar Atauri I.G., Malheiro A.C, Santos J.A.: Modelling climate change impacts on viticultural yield, phenology and stress conditions in Europe. — Glob. Change Biol. 22: 3774–3788, 2016.

    Article  Google Scholar 

  • Gamon J.A., Pearcy R.W.: Leaf movement, stress avoidance and photosynthesis in Vitis californica. — Oecologia 79: 475–481, 1989.

    Article  CAS  PubMed  Google Scholar 

  • Genty B., Briantais J.M., Baker N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. — Biochim. Biophys. Acta. 990: 87–92, 1989.

    Article  CAS  Google Scholar 

  • Gitelson A.A., Merzlyak M.N.: Non-destructive assessment of chlorophyll, carotenoid and anthocyanin content in higher plant leaves: Principles and algorithms. — In: Stamatiadis S., Lynch J.M., Shepers J.S. (ed.): Non-destructive Assessment of Chlorophyll, Carotenoids and Anthocyanin Content in Higher Plant Leaves: Principles and Algo-rithms. Remote Sensing for Agriculture and the Environment. Pp. 78–94. Ella, Greece 2004.

  • Glenn D.M., Erez A., Puterka G.J., Gundrum P.: Particle films affect carbon assimilation and yield in ‘Empire’ apple. — J. Am. Soc. Hortic. Sci. 128: 356–362, 2003.

    Google Scholar 

  • Glenn D.M., Puterka G.J.: Particle films: A new technology for agriculture. — In: Janick J. (ed.): Horticultural Reviews, Vol. 31. Pp. 1–44, John Wiley & Sons, New Jersey 2005.

    Google Scholar 

  • Glenn D.M., Puterka G.J., Drake S.R. et al.: Particle film application influences apple leaf physiology fruit yield and fruit quality. — J. Am. Soc. Hortic. Sci. 126: 175–181, 2001.

    Google Scholar 

  • Hannah L., Roehrdanz P.R., Ikegami M. et al.: Climate change, wine, and conservation. — P. Natl. Acad. Sci. USA 110: 6907–6912, 2013.

    Article  CAS  Google Scholar 

  • Heath R.L., Packer L.: Photoperoxidation in isolated chloroplasts. I Kinetics and stoichiometry of fatty acid peroxidation. — Arch. Biochem. Biophys. 125: 189–198, 1968.

    Article  CAS  PubMed  Google Scholar 

  • Iacono F., Buccella A., Peterlunger E.: Water stress and rootstock influence on leaf gas exchange of grafted and ungrafted grapevines. — Sci. Hortic.-Amsterdam 75: 27–39, 1998.

    Article  Google Scholar 

  • IPCC.: Climate Change. The Physical Science Basis. Summary for Policymakers. Working Group I Contribution to the IPCC Fifth Assessment Report. Pp. 1–28. Cambridge Univ. Press, Cambridge 2013.

    Google Scholar 

  • Irigoyen J.J., Einerich D.W., Sánchez-Diáz M.: Water stress induced changes in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativa) plants. — Plant Physiol. 84: 55–60, 1992.

    Article  CAS  Google Scholar 

  • Jifon J.L., Syvertsen J.P.: Kaolin particle film applications can increase photosynthesis and water use efficiency of ‘Ruby Red’ grapefruits leaves. — J. Am. Soc. Hortic. Sci. 128: 107–112, 2003.

    CAS  Google Scholar 

  • Jones G.V., White M.A., Cooper O.R., Storchmann K.: Climate change and global wine quality. — Climatic Change 73: 319–343, 2005.

    Article  Google Scholar 

  • Koricheva J., Larsson S., Haukioja E., Keinänen M.: Regulation of woody plant secondary metabolism by resource availability: hypothesis testing by means of meta-analysis. — Oikos 83: 212–226, 1998.

    Article  CAS  Google Scholar 

  • Kottek M., Grieser J., Beck C.: World Map of the Köppen-Geiger climate classification updated. — Meteorol Z. 15: 259–263, 2006.

    Article  Google Scholar 

  • Lichtenthaler H.K.: Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. — Methods Enzymol. 148: 350–382, 1987.

    Article  CAS  Google Scholar 

  • Marinari S., Calfapietra C., De Angelis P. et al.: Impact of elevated CO2 and nitrogen fertilization on foliar elemental composition in a short rotation poplar plantation. — Environ. Pollut. 147: 507–515, 2007.

    Article  CAS  PubMed  Google Scholar 

  • Medrano H., Escalona J.M., Bota J. et al.: Regulation of photosynthesis of C3 plants in response to progressive drought: Stomatal conductance as a reference parameter. — Ann. Bot.-London 89: 895–905, 2002.

    Article  CAS  Google Scholar 

  • Merzlyak M.N., Solovchenko A.E., Gitelson A.A.: Reflectance spectral features and non-destructive estimation of chlorophyll, carotenoid and anthocyanin content in apple fruit. — Postharvest Biol. Tec. 27: 197–211, 2003.

    Article  CAS  Google Scholar 

  • Moutinho-Pereira J., Correia C.M., Gonçalves B. et al.: Impacts of leafroll associated viruses (GLRaV-1 and-3) on the physiology of the Portuguese grapevine cultivar 'Touriga Nacional' growing under field conditions. — Ann. Appl. Biol. 160: 237–249, 2012.

    Article  CAS  Google Scholar 

  • Moutinho-Pereira J.M., Correia C.M., Gonçalves B. et al.: Leaf gas-exchange and water relations of grapevines grown in three different conditions. — Photosynthetica 42: 81–86, 2004.

    Article  Google Scholar 

  • Moutinho-Pereira J.M., Magalhães N., Gonçalves B.: Gas exchange and water relations of three Vitis vinifera L. cultivars growing under Mediterranean climate. — Photosynthetica 45: 202–207, 2007.

    Article  CAS  Google Scholar 

  • Osaki M., Shinano T., Tadano T.: Redistribution of carbon and nitrogen compound from the shoot to the harvesting organs during maturation in field crops. — Soil Sci. Plant Nutr. 37: 117–128, 1991.

    Article  CAS  Google Scholar 

  • Patakas A., Noitsakis B.: Leaf age effects on solute accumulation in water-stressed grapevines. — J. Plant Physiol. 158: 63–69, 2001.

    Article  CAS  Google Scholar 

  • Poni S., Bernizzoni F., Civardi S.: Performance and water-use efficiency (single-leaf vs. whole-canopy) of well-watered and half-stressed split-root Lambrusco grapevines grown in Po Valley (Italy). — Agr. Ecosyst. Environ. 129: 97–106, 2009.

    Article  Google Scholar 

  • Rosati A., Metcalf S.G., Buchner R.P. et al.: Physiological effects of kaolin applications in well-irrigated and waterstressed walnut and almond trees. — Ann. Bot.-London 98: 267–275, 2006.

    Article  CAS  Google Scholar 

  • Sampol B., Bota J., Riera D. et al.: Analysis of the virus-induced inhibition of photosynthesis in malmsey grapevines. — New Phytol. 160: 403–412, 2003.

    Article  CAS  Google Scholar 

  • Santos C.V., Caldeira G.: Comparative responses of Helianthus annuus plants and calli exposed to NaCl. I. Growth rate and osmotic adjustment in intact plants and calli. — J. Plant Physiol. 155: 769–777, 1999.

    Article  Google Scholar 

  • Šesták Z., Čatský J., Jarvis P.G.: Plant Photosynthetic Production. Manual of Methods. Pp. 818. Dr. W. Junk Publ., Haia 1971.

    Google Scholar 

  • Shalata A., Tal A.: The effects of salt stress on lipid peroxidation and antioxidants in the leaf of the cultivated tomato and its wild salt-tolerant relative Lycopersicon pennellii. — Physiol. Plantarum 104: 169–174, 1998.

    Article  CAS  Google Scholar 

  • Shellie K.C., King B.A.: Kaolin particle film and water deficit influence malbec leaf and berry temperature, pigments, and photosynthesis. — Am. J. Enol. Viticult. 64: 223–230, 2013.

    Article  CAS  Google Scholar 

  • Singleton V.L., Rossi J.A.J.: Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. — Am. J. Enol. Viticult. 16: 144–158, 1965.

    CAS  Google Scholar 

  • Smirnoff N.: The role of active oxygen in the response of plants to water deficit and desiccation. — New Phytol. 125: 27–58, 1993.

    Article  CAS  Google Scholar 

  • Valladares R., Pearcy R.W.: Interaction between water stress, sun-shade acclimation, heat tolerance and photoinhibition in the sclerophyll Heteromeles arbutifolia. — Plant Cell Environ. 20: 25–36, 1997.

    Article  Google Scholar 

  • von Caemmerer S., Farquhar G.D.: Some relationships between the biochemistry of photosynthesis and the gas exchange of leaves. — Planta 153: 376–387, 1981.

    Article  Google Scholar 

  • Wen P.-F., Chen J.-Y., Wan S.-B. et al.: Salicylic acid activates phenylalanine ammonia-lyase in grape berry in response to high temperature stress. — Plant Growth Regul. 55: 1–10, 2008.

    Article  CAS  Google Scholar 

  • Yadav V.K., Gupta V., Neelam Y.: Hormonal regulation of nitrate in gram (Cicer arietinum) genotypes under drought. — Indian J. Agr. Sci. 69: 592–595, 1999.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Universidade de Trás-os-Montes e Alto Douro, Apt. 1013, 5001-801, Vila Real, Portugal

    L.-T. Dinis, A. C. Malheiro, A. Luzio, H. Fraga, H. Ferreira, C. M. Correia & J. Moutinho-Pereira

  2. Associação para o Desenvolvimento da Viticultura Duriense (ADVID), Edifício Centro de Excelência da Vinha e do Vinho, Parque de Ciência e Tecnologia de Vila Real – Régia Douro Park, 5000-033, Vila Real, Portugal

    I. Gonçalves

  3. Department of Biology & CESAM – Centre for Environmental and Marine Studies, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal

    G. Pinto

Authors
  1. L.-T. Dinis
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  2. A. C. Malheiro
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  3. A. Luzio
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  4. H. Fraga
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  5. H. Ferreira
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  6. I. Gonçalves
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  7. G. Pinto
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  8. C. M. Correia
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  9. J. Moutinho-Pereira
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Corresponding author

Correspondence to L.-T. Dinis.

Additional information

Acknowledgements: The study was undertaken under the IC&DT INTERACT project–“Integrated Research in Environment, Agro- Chain and Technology”, no. NORTE-01-0145-FEDER-000017, in its line of research entitled VitalityWINE, co-financed by the European Regional Development Fund (ERDF) through NORTE 2020 and Award “Fundação Maria Rosa” to our team. The postdoctoral fellowships awarded to L.-T. Dinis (SFRH/BPD/84676/2012), A. Luzio (BPD/INTERACT/VITALITYWINE/184/2016), and G. Pinto (SFR/BPD/101669/2014) are appreciated. We would also like to thank to “Quinta do Vallado” for the collaboration and efforts in making the vineyard’s facilities available for the research and particularly to António Pinto and Daniel Gomes and BASF for collaboration.

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Dinis, LT., Malheiro, A.C., Luzio, A. et al. Improvement of grapevine physiology and yield under summer stress by kaolin-foliar application: water relations, photosynthesis and oxidative damage. Photosynthetica 56, 641–651 (2018). https://doi.org/10.1007/s11099-017-0714-3

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