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Changes in the photosynthetic characteristics and photosystem stoichiometries in wild-type and Chl b-deficient mutant rice seedlings under various irradiances

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Photosynthetica

Abstract

By using a wild-type rice (Oryza sativa L. cv. Norin No. 8) and the chlorophyll (Chl) b-deficient mutant derived from Norin No. 8 (chlorina 11), the present study monitored the oxygen evolution, contents of Chl a and b, β-carotene, and lutein in leaf and the contents of cytochrome f, and the reaction centres of photosystem I (PSI) and photosystem II (PSII) in thylakoids. The oxygen evolution, maximal quantum yield of PSII (Fv/Fm) and Chl concentration remained constant in both Norin No. 8 and chlorina 11 under 5 and 2% of full sunlight for six days. On the other hand, on the thylakoid level, the PSII reaction centre of chlorina 11 was more stable even under high irradiance, while approximately 40% decrease in levels of the PSII reaction centre occurred under 2% of full sunlight for six days. However, under such conditions, by regulating the stoichiometry of active PSII and PSI centres, the light absorption balance in both rice types was adjusted between the two photosystems. The present study attempted to examine whether the light absorption balance between PSII and PSI is altered to effectively conduct photosynthesis in the wild-type and Chl b-deficient mutant rice seedlings.

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Abbreviations

C550 :

electrochromic shift of pheophytin in the reaction centre complex of photosystem II

Chl:

chlorophyll

Cyt:

cytochrome

DCMU:

3-(3,4-dichlorophenyl)-1,1-dimethylurea

Fv :

variable Chl fluorescence yield in the dark-adapted leaves

Fm :

maximal Chl fluorescence yield in the dark-adapted leaves

Fo :

minimal Chl fluorescence yield in the dark-adapted leaves

chlorina 11:

Chl b-deficient mutant derived from Norin No. 8

LHCI:

light-harvesting Chl-protein of PSI

LHCII:

lightharvesting Chl-protein of PSII

Norin No. 8:

wild-type rice

P700 :

the reaction centre complex in photosystem I

PPFD:

photosynthetic photon flux density

PQ:

plastoquinone

PSI:

photosystem I

PSII:

photosystem II

QA :

primary quinone acceptor of PSII

QB :

secondary quinone acceptor of PSII

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Acknowledgements

I express my gratitude to my mentor, Professor Yasumaro Kamimura and Professor Emiko Maruta, for their many critical suggestions and encouragement throughout this work. I also thank Tomoyuki Nakayama, Junko Sakabe, Tomomi Shinobu, Reina Arakawa, Takeshi Katoh, Sachiko Mizukami, Ryoko Yabe, Mayo Karube, Yuko Shinomiya, Eriko Tsurumi and my other collaborators for their excellent assistance and continuous encouragement throughout this work.

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  1. Department of Biology, Faculty of Science, Toho University, Miyama 2-2-1, Funabashi, Chiba, 274-8510, Japan

    J. Yamazaki

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Yamazaki, J. Changes in the photosynthetic characteristics and photosystem stoichiometries in wild-type and Chl b-deficient mutant rice seedlings under various irradiances. Photosynthetica 48, 521–529 (2010). https://doi.org/10.1007/s11099-010-0069-5

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