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Photoprotective mechanisms in cold-acclimated and nonacclimated needles of Picea glehnii

  • Original Papers
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Photosynthetica

Abstract

The response of Picea glehnii, a cold-tolerant species in the boreal zone, to air temperature (T) was investigated for its cold-acclimated needles (i.e. the ones subjected to gradual decrease in T) and nonacclimated needles (i.e. the ones subjected to a sudden decrease in T) were compared under low temperature. Cold-acclimated needles showed a greater increase of zeaxanthin and lutein contents than nonacclimated ones, whereas the nonacclimated needles showed a greater increase of thylakoid-bound ascorbate peroxidase (tAPX) activity than cold-acclimated ones under chilling conditions (after cold acclimation). These results suggest that: (1) low T induces the increase of zeaxanthin and lutein content, and tAPX activity; (2) accumulated zeaxanthin and lutein protect needles from photooxidative stress by dissipating excess energy before the reactive oxygen species (ROS) are formed in response to a gradual decrease in T (with cold acclimation and subsequent chilling condition), and by tAPX scavenging ROS formed in the case of a sudden decrease in T (without cold acclimation and chilling condition).

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Abbreviations

A:

antheraxanthin

APX:

ascorbate peroxidase

C:

control

CA:

cold-acclimated

Chl:

chlorophyll

DM:

dry mass

Fm :

fluorescence when all PSII reaction centres are closed in dark-exposed leaves

Fo :

fluorescence of leaves in the dark when all PSII reaction centres are open

Fv :

variable fluorescence (Fv = Fm−Fo)

Fv/Fm :

quantum yield of PSII photochemistry

FM:

fresh mass

N:

nonacclimated

PPFD:

photosynthetic photon flux density

ROS:

reactive oxygen species

RWC:

relative water content

sAPX:

stromal APX

tAPX:

thylakoid-bound APX

T:

temperature

TM:

turgescent mass

V:

violaxanthin

Z:

zeaxanthin

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Acknowledgement

The Korean Research Foundation Grant (KRF-2005-214-C00127), CREST grant, and ILTS grant supported this work.

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Authors and Affiliations

  1. Cryosphere Science Research Section, Institute of Low Temp. Science, Hokkaido Univ. Sapporo, Sapporo, 060-0819, Japan

    K. Ono, A. Sumida & T. Hara

  2. Plant Ecophysiology Lab., Department of Biology, Kyungpook National Univ., Daegu, 702-701, Korea

    J. -J. Bae & Y. -S. Choo

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  1. J. -J. Bae
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  2. Y. -S. Choo
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  3. K. Ono
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  4. A. Sumida
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Correspondence to J. -J. Bae.

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Bae, J.J., Choo, Y.S., Ono, K. et al. Photoprotective mechanisms in cold-acclimated and nonacclimated needles of Picea glehnii . Photosynthetica 48, 110–116 (2010). https://doi.org/10.1007/s11099-010-0015-6

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  • DOI: https://doi.org/10.1007/s11099-010-0015-6

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