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Xanthophyll cycle activity in detached barley leaves senescing under dark and light

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Photosynthetica

Abstract

Senescence-induced changes in the xanthophyll cycle activity and chlorophyll (Chl) fluorescence parameters were compared in detached barley (Hordeum vulgare L.) leaf segments kept for 6 d in darkness or under continuous “ white light” (90 μmol m−2 s−1). Before detachment of the leaf segments, the plants were grown at periodic regime [12 h light (90 μmol m−2 s−1)/12 h dark]. The de-epoxidation state of the xanthophyll cycle pigments (DEPS) in the leaf samples was determined immediately (the actual DEPS), after 1 h of dark-adaptation (the residual DEPS), and during 14 min of a high-irradiance (HI) exposure (500 μmol m−2 s−1) (HI-induced DEPS). In the light-senescing segments, senescence was delayed pronouncedly compared to dark-senescing ones as the Chl content, the photosystem 2 photochemistry, and electron transport processes were highly maintained. Further, the actual DEPS increased, probably due to the increased mean photon dose. The HI-induced increase in the DEPS was stimulated in the light-senescing segments, whereas it was slowed down in the dark-senescing ones. However, after the 14 min HI-exposure of the dark-senescing segments the HI-induced DEPS was not markedly lower than in the mature leaves, which indicated the maintenance of the xanthophyll cycle operation.

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Abbreviations

A:

antheraxanthin

Car:

sum of carotenoids

Chl:

chlorophyll

DEPS:

de-epoxidation state of the xanthophyll cycle pigments

HI:

high irradiance

MGDG:

monogalactosyldiacylglycerol

NPQ:

non-photochemical chlorophyll fluorescence quenching

PAR:

photosynthetically active radiation

PS2:

photosystem 2

V:

violaxanthin

Z:

zeaxanthin

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Authors and Affiliations

  1. Department of Experimental Physics, Palacký University, Tř. Svobody 26, 771 46, Olomouc, Czech Republic

    M. Špundová, K. Strzałka & J. Nauš

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  1. M. Špundová
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  2. K. Strzałka
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  3. J. Nauš
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Špundová, M., Strzałka, K. & Nauš, J. Xanthophyll cycle activity in detached barley leaves senescing under dark and light. Photosynthetica 43, 117–124 (2005). https://doi.org/10.1007/s11099-005-7124-7

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  • DOI: https://doi.org/10.1007/s11099-005-7124-7

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