Abstract
In order to maximize yield, Spathiphyllum, an ornamental plant, was cultured in vitro in novel culture vessels termed Vitron. The best growth was obtained by culturing plantlets on sugar-free liquid medium under CO2 enrichment (3 000 µmol mol−1 24 h−1 d−1) at a low photon flux density (PPFD of 45 µmol m−2 s−1), suggesting that the novel Vitron culture system is suitable for the photoautotrophic micropropagation of Spathiphyllum.
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Aitken-Christie, J., Kozai, T., Smith, M.A.L. (ed.): Automation and Environmental Control in Plant Tissue Culture. — Kluwer Academic Publ., Dordrecht — Boston — London 1995.
Buddendorf-Joosten, J.M.C., Woltering, E.J.: Components of the gaseous environment and their effects on plant growth and development in vitro. — In: Lumsden, P.J., Nicholas, J.R., Davies, W.J. (ed.): Growth and Development of Plants in Culture. Pp. 95–125. Kluwer Academic Publ., Dordrecht — Boston — London 1994.
Desjardins, Y.: Photosynthesis in vitro — on the factors regulating CO2 assimilation in micropropagation systems. — Acta Hort. 393: 45–61, 1995.
Fujiwara, K., Kozai, T., Watanabe, I.: Development of a photoautotrophic tissue culture system for shoots and/or plantlets at rooting and acclimatization stages. — Acta Hort. 230: 153–158, 1988.
Infante, R., Magnanini, E., Righetti, B.: The role of light and CO2 in optimising the conditions for shoot proliferation of Actinidia deliciosa in vitro. — Physiol. Plant. 77: 191–195, 1989.
Jackson, M.B., Abbott, A.J., Belcher, A.R., Hall, K.C., Butler, R., Cameron, J.: Ventilation in tissue cultures and effects of poor aeration on ethylene and carbon dioxide accumulation, oxygen depletion and explant development. — Ann. Bot. 67: 229–237, 1991.
Kool, L.T., Keng, C.L., Hoe, C.T.K.: In vitro rooting of Sentang shoots (Azadirachta excelsa L.) and acclimatization of plantlets. — In Vitro cell. develop. Biol. Plant. 35: 396–400, 1999.
Kozai, T.: Micropropagation under photoautotrophic conditions. — In: Debergh, P., Zimmerman, R. (ed.): Micropropagation. Technology and Application. Pp. 447–469. Kluwer Academic Publ., Dordrecht — Boston — London 1991a.
Kozai, T.: Photoautotrophic micropropagation. — In Vitro cell. develop. Biol. Plant. 27: 47–51, 1991b.
Kozai, T., Iwanami, Y., Fujiwara, K.: [Environment control for mass propagation of tissue cultured plantlets. (1) Effects of CO2 enrichment on the plantlet growth during the multiplication stage.] — Plant Tissue Cult. Lett. 4: 22–26, 1987a. [In Jap.]
Kozai, T., Kubota, C., Jeong, B.R.: Environmental control for large-scale production of plants through in vitro techniques. — Plant Cell Tissue Organ Cult. 51: 49–56, 1997.
Kozai, T., Oki, H., Fujiwara, K.: Effect of CO2 enrichment and sucrose concentration under high photosynthetic photon fluxes and on growth of tissue cultured Cymbidium plantlets during preparation. — Plant Microprop. Hort. Ind. 11: 135–141, 1987b.
Kozai, T., Tanaka, K., Jeong, B.R., Fujiwara, K.: Effect of relative humidity in the culture vessels on the growth and shoot elongation of potato (Solanum tuberosum L.) plantlets in vitro. — J. jap. Soc. hort. Sci. 62: 413–417, 1993.
Lees, R.P., Evans, E.H., Nicholas, J.R.: Photosynthesis in Clematis ‘The President’ during growth in vitro and subsequent in vivo acclimatization. — J. exp. Bot. 42: 605–610, 1991.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. — Physiol. Plant. 15: 473–497, 1962.
Nagae, S., Takamura, T., Tanabe, T., Murakami, A., Murakami, K., Tanaka, M.: In vitro shoot development of Eucalyptus citriodora on rockwool in the film culture vessel under CO2 enrichment. — J. Forest Res. 1: 227–230, 1996.
Nguyen, Q.T., Kozai, T., Heo, J., Thai, D.X.: Photoautotrophic growth response of in vitro cultured coffee plantlets to ventilation methods and photosynthetic photon fluxes under carbon dioxide enriched condition. — Plant Cell Tissue Organ Cult. 66: 217–225, 2001.
Nguyen, Q.T., Kozai, T., Niu, G., Nguyen, V.U.: Photosynthetic characteristics of coffee (Coffea arbusta) plantlets in vitro in response to different CO2 concentration and light intensities. — Plant Cell Tissue Organ Cult. 55: 133–139, 1999.
Nhut, D.T., Hong, L.T.A., Watanabe, H., Goi, M., Tanaka, M.: In vitro growth of banana plantlets cultured under red and blue light-emitting diode (LED) irradiation source. — Acta Hort. 575: 117–123, 2002a.
Nhut, D.T., Takamura, T., Watanabe, H., Murakami, A., Murakami, K., Tanaka, M.: Sugar-free micropropagation of Eucalyptus citriodora using light-emitting diodes (LEDs) and film-rockwool culture system. — Environ. Contr. Biol. 40: 147–155, 2002b.
Nhut, D.T., Takamura, T., Watanabe, H., Okamoto, K., Tanaka, M.: Responses of strawberry plantlets cultured in vitro under superbright red and blue light-emiiting diodes (LEDs). — Plant Cell Tissue Organ Cult. 73: 43–52, 2003.
Reuther, G.: Stimulation of the photoautotrophy of in vitro plants. — Acta Hort. 300: 59–75, 1991.
Sheen, J.: Metabolic repression of transcription in higher plants. — Plant Cell 2: 1027–1038, 1990.
Solarova, J.: Photosynthesis of plant regenerants. Diurnal variation in CO2 concentration in cultivation vessels resulting from plantlets photosynthetic activity. — Photosynthetica 23: 100–107, 1989.
Solarova, J., Souckova, D., Ullmann, J., Pospisilova, J.: In vitro culture: environmental conditions and plantlets growth as affected by vessel and stopper types. — HortScience 23: 51–58, 1996.
Tanaka, M.: Disposable film culture vessels. — In: Bajaj, Y.P.S. (ed.): Biotechnology in Agriculture and Forestry. Pp. 212–228. Springer-Verlag, Berlin 1991a.
Tanaka, M., Goi, M., Higashiura, T.: A novel disposable film culture vessel in micropropagation. — Acta Hort. 226: 663–670, 1988a.
Tanaka, M., Hirano, T., Goi, M., Higashiura, T., Sasahara, H., Murasaki, K.: Practical application of a novel disposable film culture vessel in micropropagation. — Acta Hort. 300: 77–84, 1991b.
Tanaka, M., Ikeda, M., Fukai, S., Goi, M.: Effect of different films used for film culture vessels on plantlet development of Phalaenopsis and Cymbidium. — Acta Hort. 319: 225–230, 1992a.
Tanaka, M., Jinno, K., Goi, M., Higashiura, T.: The use of disposable fluorocarbon polymer film culture vessel in micropropagation. — Acta Hort. 230: 73–80, 1988b.
Tanaka, M., Nagae, S., Fukai, S., Goi, M.: Growth of tissue-cultured Spathiphyllum on rockwool in a novel film culture vessel under high CO2. — Acta Hort. 314: 139–146, 1992b.
Tanaka, M.; Nagae, S., Takamura, T., Kusanagi, N., Ujike, M., Goi, M.: Efficiency and application of film culture system in the in vitro production of plantlets in some horticultural crops. — J. Soc. high Technol. Agr. 8: 280–285, 1996.
Tanaka, M., Yap, D.C.H., Ng, C.K.Y., Hew, C.S.: The physiology of Cymbidium plantlets cultured in vitro under conditions of high carbon dioxide and low photosynthetic photon density. — J. Hort. Sci. Biotech. 74: 632–638, 1999.
Ziv, M., Meir, G., Halevy, A.H.: Factors influencing the production of hardened glaucous carnation plantlets in vitro. — Plant Cell Tissue Organ Cult. 2: 55–63, 1983.
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Teixeira Da Silva, J.A., Giang, D.D.T. & Tanaka, M. Photoautotrophic micropropagation of Spathiphyllum . Photosynthetica 44, 53–61 (2006). https://doi.org/10.1007/s11099-005-0158-z
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DOI: https://doi.org/10.1007/s11099-005-0158-z