Skip to main content
SpringerLink
Log in

Effect of ectomycorrhizal infection on growth and photosynthetic characteristics of Pinus densiflora seedlings grown under elevated CO2 concentrations

  • Published:
Photosynthetica

Abstract

The effect of ectomycorrhizal Pisolithus tinctorius (Pt) infection was studied on the growth and photosynthetic characteristics of Pinus densiflora seedlings grown at ambient (360 µmol mol−1, AC) and elevated (720 µmol mol−1, EC) CO2 concentrations. After 18 weeks, Pt inoculation had led to significantly increased dry mass and stem diameter of P. densiflora at both CO2 concentrations, relative to non-inoculated seedlings. Moreover, EC significantly increased the ectomycorrhizal development. The phosphate content in needles inoculated with Pt was about three times higher than without inoculation at both CO2 concentrations. The PAR saturated net photosynthetic rates (P sat) of P. densiflora inoculated with Pt were clearly higher than for control seedlings at both CO2 concentrations, and the maximum net photosynthetic rate (P N) at saturated CO2 concentration (P max) was higher than in controls. Moreover, the carboxylation efficiency (CE) and RuBP regeneration rate of the P N/C i curve for P. densiflora inoculated with Pt were significantly higher than for non-inoculated seedlings at both CO2 concentrations, especially at EC. The water use efficiency (WUE) of seedlings inoculated with Pt grown at EC was significantly raised. Allocation of photosynthates to roots was greater in Pt inoculated pine seedlings, because of the enhanced activity of ectomycorrhiza associated with seedlings at EC. Moreover, P N of non-inoculated seedlings grown for 18 weeks at EC tended to be down regulated; in contrast, Pt inoculated seedlings showed no down-regulation at EC. The activity of ectomycorrhiza may therefore be enhanced physiological function related to water and phosphate absorption in P. densiflora seedlings at EC.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Reference

  • Adam, N.R., Wall, G.W., Kimball, B.A., Idso, S.B., Webber, A.N.: Photosynthetic down-regulation over long-term CO2 enrichment in leaves of sour orange (Citrus aurantium) trees. — New Phytol. 163: 341–347, 2004.

    Article  Google Scholar 

  • Allen, M.F.: The Ecology of Mycorrhizae. — Cambridge University Press, New York 1991.

    Google Scholar 

  • Allen, M.F. (ed.): Mycorrhizal Functioning. — Chapman & Hall, New York 1992.

    Google Scholar 

  • Berntson, G.M., Bazzaz, F.A.: Below-ground positive and negative feedbacks on CO2 growth enhancement. — Plant Soil 187: 119–131, 1996.

    Article  Google Scholar 

  • Bolan, N.S.: A critical review on the role of mycorrhizal fungi in the uptake of phosphorus by plants. — Plant Soil 134: 189–207, 1991.

    Article  Google Scholar 

  • Ceulemans, R., Mousseau, M.: Effects of elevated atmospheric CO2 on woody plants. — New Phytol. 127: 425–446, 1994.

    Google Scholar 

  • Conroy, J.P., Smillie, R.M., Kuppers, M., Bevege, D.J., Barlow, E.W.: Chlorophyll a fluorescence and photosynthetic and growth response of Pinus radiata to phosphorus deficiency, drought stress, and high CO2. — Plant Physiol. 81: 423–429, 1986.

    Google Scholar 

  • Dunabeitia, M.K., Hormilla, S., Garcia-Plazaola, J.I., Txarterina, K. Arteche, U., Becerril, J.M.: Differential responses of three fungal species to environmental factors and their role in the mycorrhization of Pinus radiata D. Don. — Mycorrhiza 14: 11–18, 2004.

    Article  PubMed  Google Scholar 

  • Eguchi, N., Fukatsu, E., Funada, R., Tobita, H., Kitao, M., Maruyama, Y., Koike, T.: Changes in morphology, anatomy, and photosynthetic capacity of needles of Japanese larch (Larix kaempferi) seedlings grown in high CO2 concentrations. — Photosynthetica 42: 173–178, 2004.

    Article  Google Scholar 

  • Farquhar, G.D., Caemmerer, S. von, Berry, J.A.: A biochemical model of photosynthetic CO2 assimilation in leaves of C3 species. — Planta 149: 78–90, 1980.

    Article  Google Scholar 

  • Farquhar, G.D., Sharkey, T.D.: Stomatal conductance and photosynthesis. — Annu. Rev. Plant Physiol. 33: 317–345, 1982.

    Article  Google Scholar 

  • Farrar, J.F., Williams, M.L.: The effects of increased atmospheric carbon dioxide and temperature on carbon partitioning, source-sink relations and respiration. — Plant Cell Environ. 14: 819–830, 1991.

    Google Scholar 

  • Field, C., Merino, J., Mooney, H.A.: Compromises between water-use efficiency and nitrogen-use efficiency in five species of California evergreens. — Oecologia 60: 384–389, 1983.

    Article  Google Scholar 

  • Finlay, R.D.: Uptake and translocation of nutrients by ectomycorrhizal fungal mycelia. — In: Read, D.J., Lewis, D.H., Fitter, A.H., Alexander, I.J. (ed.): Mycorrhizas in Ecosystems. Pp. 92–97. CAB International, Wallingford 1992.

    Google Scholar 

  • Fredeen, A.L., Raab, T.K., Rao, I.M., Terry, N.: Effects of phosphorus nutrition on photosynthesis in Glycine max (L.) Merr. — Planta 181: 399–405, 1990.

    Article  Google Scholar 

  • Gavito, M.E., Curtis, P.S., Mikkelsen, T.N., Jakobsen, I.: Atmospheric CO2 and mycorrhiza effects on biomass allocation and nutrient uptake of nodulated pea (Pisum sativum L.) plants. — J. exp. Bot. 51: 1931–1938, 2000.

    PubMed  Google Scholar 

  • Griffin, K.L., Tissue, D.T., Turnbull, M.H., Whitehead, D.: The onset of photosynthetic acclimation to elevated CO2 partial pressure in field-grown Pinus radiata D. Don. after 4 years. — Plant Cell Environ. 23: 1089–1098, 2000.

    Article  Google Scholar 

  • Guehl, J.M., Mousain, D., Falconnet, G., Gruez, J.: Growth, carbon dioxide assimilation capacity and water-use efficiency of Pinus pinea L. seedlings inoculated with different ectomycorrhizal fungi. — Ann. Sci. forest. 47: 91–100, 1990.

    Google Scholar 

  • Harley, P.C., Sharkey, T.D.: An improved model of C3 photosynthesis at high CO2: Reversed O2 sensitivity explained by lack of glycerate reentry into the chloroplast. — Photosynth. Res. 27: 169–178, 1991.

    Google Scholar 

  • Hensen, C.W., Lynch, J., Ottosen, C.O.: Response to phosphorus availability during vegetative and reproductive growth of Chrysanthemum: I. Whole-plant carbon dioxide exchange. — J. amer. Soc. horticult. Sci. 123: 215–222, 1998.

    Google Scholar 

  • Idso, S.B., Kimball, B.A.: Downward regulation of photosynthesis and growth at high CO2 levels. No evidence for either phenomenon in three-year study of sour orange trees. — Plant Physiol. 96: 990–992, 1991.

    Google Scholar 

  • Ineichen, K., Wiemken, V., Wiemken, A.: Shoots, roots and ectomycorrhizal formation of pine seedlings at elevated atmospheric carbon dioxide. — Plant Cell Environ. 18: 703–707, 1995.

    Google Scholar 

  • IPCC: Climate Change 1995: The Science of Climate Change, Summary for Policymakers. — Cambridge University Press, New York 1996.

  • Jach, M.E., Ceulemans, R.: Effects of season, needle age and elevated atmospheric CO2 on photosynthesis in Scots pine (Pinus sylvestris). — Tree Physiol. 20: 145–157, 2000.

    PubMed  Google Scholar 

  • Kirschbaum, M.U.F., Tompkins, D.: Photosynthetic responses to phosphorus nutrition in Eucalyptus grandis seedlings. — Aust. J. Plant Physiol. 17: 527–535, 1990.

    Google Scholar 

  • Koike, T.: Effects of CO2 in interaction with temperature and soil fertility on the foliar phenology of alder, birch, and maple seedlings. — Can. J. Bot. 73: 149–157, 1995.

    Google Scholar 

  • Koike, T., Yazaki, K., Funada, R., Kitao, M., Maruyama, Y., Takahashi, K., Maximov, T.C., Ivanov, B.I.: Photosynthetic characteristics of Dahurian larch, Scotch pine and white birch seedlings native to eastern Siberia raised under elevated CO2. — Eurasian J. Forest Res. 1: 31–37, 2000.

    Google Scholar 

  • Lambers, H., Chapin, F.S., III, Pons, T.L.: Plant Physiological Ecology. — Springer-Verlag, New York 1998.

    Google Scholar 

  • Lewis, J.D., Griffin, K.L., Thomas, R.B., Strain, B.R.: Phosphorus supply affects the photosynthetic capacity of loblolly pine grown in elevated carbon dioxide. — Tree Physiol. 14: 1229–1244, 1994.

    PubMed  Google Scholar 

  • Li, J.C.R.: Analysis of variance versus Chi-square test. — In: Statistical Inference. Pp. 471–484. Edwards Brothers, Ann Arbor 1964.

    Google Scholar 

  • Marx, D.H., Cordell, C.E. Kenney, D.S., Mexal, J.G., Artman, J.D., Riffle, J.W., Molina, R.J.: Commercial vegetative inoculum of Pisolithus tinctorius and inoculation techniques for development of ectomycorrhizae on bare-root seedlings. — Forest Sci. Monogr. 25: 1–101, 1984.

    Google Scholar 

  • Moroto, M., Mashimo, Y., Harata, Y.: [Soil properties and growth of Japanese red pine (Pinus densiflora) in the hilly and low-mountainous region of central Japan.] — J. jap. Forestry Soc. 69: 371–378, 1987. [In Jap.]

    Google Scholar 

  • Mousseau, M., Dufrene, E., El Kohen, A., Epron, D., Godard, D., Liozon, R., Pontailler, J.Y., Saugier, B.: Growth strategy and tree response to elevated CO2: A comparison of beech (Fagus sylvatica) and sweet chestnut (Castanea sativa Mill.). — In: Koch, G.W., Mooney, G.A. (ed.): Carbon Dioxide and Terrestrial Ecosystems. Pp. 71–86. Academic Press, San Diego — New York — Boston — London — Sydney — Tokyo — Toronto 1996.

    Google Scholar 

  • Norby, R.J., O’Neill, E.G., Hood, W.G., Luxmoore, R.J.: Carbon allocation, root exudation and mycorrhizal colonization of Pinus echinata seedlings grown under CO2 enrichment. — Tree Physiol. 3: 203–210, 1987.

    PubMed  Google Scholar 

  • Norby, R.J., Wullschleger, S.D., Gunderson, C.A.: Tree responses to elevated CO2 and implications for forests. — In: Koch, G.A., Mooney, H.A. (ed.): Carbon Dioxide and Terrestrial Ecosystems. Pp. 1–21. Academic Press, San Diego — New York — Boston — London — Sydney — Tokyo — Toronto 1996.

    Google Scholar 

  • Quoreshi, A.M.: Nutritional preconditioning and ectomycorrhizal formation of Picea mariana (Mill.) B.S.P. seedlings. — Eurasian J. Forest Res. 6: 1–63, 2003.

    Google Scholar 

  • Quoreshi, A.M., Maruyama, Y., Koike, T.: The role of mycorrhiza in forest ecosystems under CO2-enriched atmosphere. — Eurasian J. Forest Res. 6-2: 171–176, 2003.

    Google Scholar 

  • Reid, C.P.P., Kidd, F.A., Ekwebelam, S.A.: Nitrogen nutrition, photosynthesis and carbon allocation in ectomycorrhizal pine. — Plant Soil 71: 415–431, 1983.

    Google Scholar 

  • Rey, A., Jarvis, P.G.: Growth response of young birch trees (Betula pendula Roth.) after four and a half years of CO2 exposure. — Ann. Bot. 80: 809–816, 1997.

    Article  Google Scholar 

  • Rogers, A., Fischer, B.U., Bryant, J., Frehner, M., Blum, H., Raines, C.A., Long, S.P.: Acclimation of photosynthesis to elevated CO2 under low-nitrogen nutrition is affected by the capacity for assimilate utilization. Perennial ryegrass under free-air CO2 enrichment. — Plant Physiol. 118: 683–689, 1998.

    Article  PubMed  Google Scholar 

  • Rouhier, H., Read, D.J.: Plant and fungal responses to elevated atmospheric carbon dioxide in mycorrhizal seedlings of Pinus sylvestris. — Environ. exp. Bot. 40: 237–246, 1998.

    Article  Google Scholar 

  • Rouhier, H., Read, D.J.: Plant and fungal responses to elevated atmospheric CO2 in mycorrhizal seedlings of Betula pendula. — Environ. exp. Bot. 42: 231–241, 1999.

    Article  Google Scholar 

  • Rousseau, J.V.D., Reid, C.P.P.: Effects of phosphorus and ectomycorrhizas on the carbon balance of loblolly pine seedlings. — Forest Sci. 36: 101–112, 1990.

    Google Scholar 

  • Runion, G.B., Mitchell, R.J., Rogers, H.H., Prior, S.A., Counts, T.K.: Effects of nitrogen and water limitation and elevated atmospheric CO2 on ectomycorrhiza of longleaf pine. — New Phytol. 137: 681–689, 1997.

    Article  Google Scholar 

  • Seegmuller, S., Rennenberg, H.: Interactive effects of mycorrhization and elevated carbon dioxide on growth of young pedunculate oak (Quercus robur L.) trees. — Plant Soil 167: 325–329, 1994.

    Article  Google Scholar 

  • Sharkey, T.D.: Photosynthesis in intact leaves of C3 plants: physics, physiology and rate limitations. — Bot. Rev. 51: 53–105, 1985a.

    Google Scholar 

  • Sharkey, T.D.: Photosynthesis of cotton plants exposed to elevated levels of carbon dioxide in the field. — Photosynth. Res. 12: 191–203, 1985b.

    Google Scholar 

  • Sharkey, T.D., Socias, X., Loreto, F.: CO2 effects on photosynthetic and product synthesis and feedback. — In: Alscher, R.G., Wellburn, A.R. (ed.): Plant Responses to the Gaseous Environment. Pp. 55–78. Chapman & Hall, London — New York 1994.

    Google Scholar 

  • Smith, S.E., Read, D.J.: Mycorrhizal Symbiosis. — Academic Press, San Diego 1997.

    Google Scholar 

  • Staddon, P.L., Fitter, A.H., Robinson, D.: Effects of mycorrhizal colonization and elevated atmospheric carbon dioxide on carbon fixation and below-ground carbon partitioning in Plantago lanceolata. — J. exp. Bot. 50: 853–860, 1999.

    Article  Google Scholar 

  • Stitt, M.: Rising CO2 levels and their potential significance for carbon flow in photosynthetic cells. — Plant Cell Environ. 14: 741–762, 1991.

    Google Scholar 

  • Terashima, I.: Anatomy of non-uniform leaf photosynthesis. — Photosynth. Res. 31: 195–212, 1992.

    Article  Google Scholar 

  • Tissue, D.T., Thomas, R.B., Strain, B.R.: Growth and photosynthesis of loblolly pine (Pinus taeda) after exposure to elevated CO2 for 19 months in the field. — Tree Physiol. 16: 49–59, 1996.

    PubMed  Google Scholar 

  • Vogel, C.S., Curtis, P.S.: Leaf gas exchange and nitrogen dynamics of N2-fixing field-grown Alnus glutinosa under elevated atmospheric CO2. — Global Change Biol. 1: 55–61, 1995.

    Google Scholar 

  • Wardlaw, I.F.: The control of carbon partitioning in plants. — New Phytol. 116: 341–381, 1990.

    Google Scholar 

  • Wright, D.P., Scholes, J.D., Read, D.J., Rolfe, S.A.: Changes in carbon allocation and expression of carbon transporter genes in Betula pendula Roth. colonized by the ectomycorrhizal fungus Paxillus involutus (Batsch) Fr. — Plant Cell Environ. 23: 39–49, 2000.

    Google Scholar 

Download references

Author information

Author notes

  1. A. M. Quoreshi

    Present address: Faculty of Forestry, University of Laval Sainte-Foy Quebec, GIK 7P4, Canada

Authors and Affiliations

  1. Graduate School of Agriculture, Hokkaido University, Sapporo, 060-8589, Japan

    D. S. Choi

  2. Hokkaido Research Center, Forestry and Forest Products Research Institute, Sapporo, 062-8516, Japan

    A. M. Quoreshi & Y. Maruyama

  3. Division of Life Science, Kyung Hee University, Yongin, 449-701, Korea

    H. O. Jin

  4. Hokkaido University Forests, FSC, Sapporo, 060-8589, Japan

    T. Koike

Authors
  1. D. S. Choi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. M. Quoreshi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Y. Maruyama
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. H. O. Jin
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. T. Koike
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to T. Koike.

Additional information

This study was partly sponsored by the Ministry of Education, Sport, Culture, Science and Technology of Japan (RR2002, Basic Research B and Sprout study).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Choi, D.S., Quoreshi, A.M., Maruyama, Y. et al. Effect of ectomycorrhizal infection on growth and photosynthetic characteristics of Pinus densiflora seedlings grown under elevated CO2 concentrations. Photosynthetica 43, 223–229 (2005). https://doi.org/10.1007/s11099-005-0037-7

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11099-005-0037-7

Additional key words

Search

Navigation