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Cerium Oxide Nanoparticles Inhibit Adipogenesis in Rat Mesenchymal Stem Cells: Potential Therapeutic Implications

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Abstract

Purpose

Cerium oxide nanoparticles (nanoceria, NC) have extraordinary antioxidant activity that made them suitable as a therapeutic agent for several diseases where reactive oxygen species (ROS) act by impairing the normal redox balance. Among different functions, it has been proven that ROS are cellular messengers involved in the adipogenesis: we thus investigated the implication of NC administration in the potential inhibition of adipogenic differentiation of mesenchymal stem cells (MSCs) used as a model of adipogenesis.

Methods

We evaluated cytotoxic effects and adipogenic maturation of mesenchymal stem cells following in vitro NC administration, both at gene and at phenotype level.

Results

Overall, our results demonstrated that NC efficiently inhibit the maturation of MSCs toward adipocytes owing to their ability to reduce the production of the ROS necessary during adipogenesis.

Conclusions

These findings, even if preliminary, represent an important step toward the potential pharmaceutical application of NC in the treatment of obesity.

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Abbreviations

MSC:

Mesenchymal stem cell

NC:

Nanoceria

qRT-PCR:

Quantitative real time reverse transcriptase-polymerase chain reaction

ROS:

Reactive oxygen species

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Authors and Affiliations

  1. Istituto Italiano di Tecnologia, Center for Micro-BioRobotics @SSSA, Viale Rinaldo Piaggio 34, 56025, Pontedera, Pisa, Italy

    Antonella Rocca, Virgilio Mattoli, Barbara Mazzolai & Gianni Ciofani

  2. Scuola Superiore Sant’Anna, The BioRobotics Institute, Viale Rinaldo Piaggio 34, 56025, Pontedera, Pisa, Italy

    Antonella Rocca

Authors
  1. Antonella Rocca
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  2. Virgilio Mattoli
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  3. Barbara Mazzolai
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  4. Gianni Ciofani
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Corresponding authors

Correspondence to Antonella Rocca or Gianni Ciofani.

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Rocca, A., Mattoli, V., Mazzolai, B. et al. Cerium Oxide Nanoparticles Inhibit Adipogenesis in Rat Mesenchymal Stem Cells: Potential Therapeutic Implications. Pharm Res 31, 2952–2962 (2014). https://doi.org/10.1007/s11095-014-1390-7

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  • DOI: https://doi.org/10.1007/s11095-014-1390-7

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