Skip to main content
SpringerLink
Log in

Characterization of a Nanoparticulate Drug Delivery System Using Scanning Ion Occlusion Sensing

  • Research Paper
  • Published:
Pharmaceutical Research Aims and scope Submit manuscript

ABSTRACT

Purpose

To explore the application of scanning ion occlusion sensing (SIOS) as a novel technology for characterization of nanoparticles.

Methods

Liposomes were employed as model nanoparticles. The size distribution of the liposomes was measured by both SIOS and dynamic light scattering (DLS). Particle number concentration was determined based on particle translocation rate. The ability of SIOS and DLS to resolve bimodal samples was evaluated by measuring a mixture of 217 and 355 nm standard nanoparticles. Opsonization of liposomes by plasma was also studied using SIOS.

Results

SIOS was shown to measure the size of different liposomes with higher sensitivity than DLS and it requires a smaller sample volume than DLS. With appropriate calibration, SIOS could be used to determine particle number concentrations. In comparison, SIOS analysis of the mixture showed accurate resolution of the population as a bimodal distribution over a wide range of number ratios of the particles. SIOS could detect plasma opsonization of liposomes by demonstrating a increase in particle size and also changes in the particle translocation rate.

Conclusion

SIOS is a useful technology for nanoparticle characterization. It shows some advantages over DLS and is clearly a useful tool for the study of nanoparticle drug delivery systems.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

REFERENCES

  1. Bootz A, Vogel V, Schubert D, Kreuter J. Comparison of scanning electron microscopy, dynamic light scattering and analytical ultracentrifugation for the sizing of poly(butyl cyanoacrylate) nanoparticles. Eur J Pharm Biopharm. 2004;57:369–75.

    Article  PubMed  CAS  Google Scholar 

  2. Krauel K, Girvan L, Hook S, Rades T. Characterisation of colloidal drug delivery systems from the naked eye to Cryo-FESEM. Micron. 2007;38:796–803.

    Article  PubMed  CAS  Google Scholar 

  3. Husain A, Hamielec AE, Vlachopoulos J. Particle size analysis using size exclusion chromatography. Size exclusion chromatography (GPC). Am Chem Soc. 1980;138:47–75.

    CAS  Google Scholar 

  4. Xu X, Caswell KK, Tucker E, Kabisatpathy S, Brodhacker KL, Scrivens WA. Size and shape separation of gold nanoparticles with preparative gel electrophoresis. J Chromatogr A. 2007;1167:35–41.

    Article  PubMed  CAS  Google Scholar 

  5. Liu L. Application of ultrasound spectroscopy for nanoparticle sizing in high concentration suspensions: A factor analysis on the effects of concentration and frequency. Chem Eng Sci. 2009;64:5036–42.

    Article  CAS  Google Scholar 

  6. Filipe V, Hawe A, Jiskoot W. Critical evaluation of nanoparticle tracking analysis (NTA) by NanoSight for the measurement of nanoparticles and protein aggregates. Pharm Res. 2010;27:796–810.

    Article  PubMed  CAS  Google Scholar 

  7. Henriquez RR, Ito T, Sun L, Crooks RM. The resurgence of Coulter counting for analyzing nanoscale objects. Analyst. 2004;129:478–82.

    Article  PubMed  CAS  Google Scholar 

  8. Sowerby SJ, Broom MF, Petersen GB. Dynamically resizable nanometre-scale apertures for molecular sensing. Sensors Actuators B Chem. 2007;123:325–30.

    Article  Google Scholar 

  9. Willmott GR, Moore PW. Reversible mechanical actuation of elastomeric nanopores. Nanotechnology. 2008;19:475504.

    Article  PubMed  CAS  Google Scholar 

  10. Willmott GR, Vogel R, Yu SSC, Groenewegen LG, Roberts GS, Kozak D, et al. Use of tunable nanopore blockade rates to investigate colloidal dispersions. J Phys.: Condens Matter. 2010;22:454116.

    Google Scholar 

  11. Vogel R, Willmott G, Kozak D, Roberts GS, Anderson W, Groenewegen L, et al. Quantitative sizing of nano/microparticles with a tunable elastomeric pore sensor. Anal Chem. 2011;83:3499–3506.

    Google Scholar 

  12. Roberts GS, Kozak D, Anderson W, Broom MF, Vogel R, Trau M. Tunable nano/micropores for particle detection and discrimination: Scanning ion occlusion spectroscopy. Small. 2010;6:2653–8.

    Article  PubMed  CAS  Google Scholar 

  13. Torchilin VP. Recent advances with liposomes as pharmaceutical carriers. Nat Rev Drug Discovery. 2005;4:145–60.

    Article  CAS  Google Scholar 

  14. Kozak D, Anderson W, Vogel R, Trau M. Advances in resistive pulse sensors: devices bridging the void between molecular and microscopic detection. Nano Today. 2011;6:531–45.

    Article  PubMed  CAS  Google Scholar 

  15. Mayer LD, Hope MJ, Cullis PR. Vesicles of variable sizes produced by a rapid extrusion procedure. BBA - Biomembranes. 1986;858:161–8.

    Article  PubMed  CAS  Google Scholar 

  16. Hunter DG, Frisken BJ. Effect of extrusion pressure and lipid properties on the size and polydispersity of lipid vesicles. Biophys J. 1998;74:2996–3002.

    Article  PubMed  CAS  Google Scholar 

  17. Shekunov BY, Chattopadhyay P, Tong HHY, Chow AHL. Particle size analysis in pharmaceutics: principles, methods and applications. Pharm Res. 2007;24:203–27.

    Article  PubMed  CAS  Google Scholar 

  18. Roberts GS, Yu S, Zeng Q, Chan LCL, Anderson W, Colby AH, et al. Tunable pores for measuring concentrations of synthetic and biological nanoparticle dispersions. Biosens Bioelectron. 2012;31:17–25.

    Google Scholar 

  19. Epstein H, Afergan E, Moise T, Richter Y, Rudich Y, Golomb G. Number-concentration of nanoparticles in liposomal and polymeric multiparticulate preparations: empirical and calculation methods. Biomaterials. 2006;27:651–9.

    Article  PubMed  CAS  Google Scholar 

  20. Tscharnuter W. Photon correlation spectroscopy in particle sizing. In: Meyers RA, editor. Encyclopedia of analytical chemistry. Chichester: John Wiley & Sons Ltd; 2000. p. 5469–85.

    Google Scholar 

  21. Frantzen CB, Ingebrigtsen L, Skar M, Brandl M. Assessing the accuracy of routine photon correlation spectroscopy analysis of heterogeneous size distributions. AAPS PharmSciTech. 2003;4:62–70.

    Article  Google Scholar 

  22. Karmali PP, Simberg D. Interactions of nanoparticles with plasma proteins: Implication on clearance and toxicity of drug delivery systems. Expert Opinion on Drug Delivery. 2011;8:343–57.

    Article  PubMed  CAS  Google Scholar 

Download references

ACKNOWLEDGMENTS AND DISCLOSURES

The research was conducted during the tenure of a Health Sciences Career Development Award from the University of Otago to Lin Yang. The authors report no conflicts of interest.

Author information

Authors and Affiliations

  1. School of Pharmacy, University of Otago, P.O. Box 56, Dunedin, 9054, New Zealand

    Lin Yang & Ian G. Tucker

  2. Private Bag 808 Picton, Marlborough, New Zealand

    Murray F. Broom

Authors
  1. Lin Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Murray F. Broom
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ian G. Tucker
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Lin Yang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yang, L., Broom, M.F. & Tucker, I.G. Characterization of a Nanoparticulate Drug Delivery System Using Scanning Ion Occlusion Sensing. Pharm Res 29, 2578–2586 (2012). https://doi.org/10.1007/s11095-012-0788-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11095-012-0788-3

KEY WORDS

Search

Navigation