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Evidence of Deep Percutaneous Penetration Associated with Anti-Inflammatory Activity of Topically Applied Helicteres gardneriana Extract: A Photoacoustic Spectroscopy Study

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ABSTRACT

Purpose

To apply the photoacoustic spectroscopy technique to investigate the penetration rate of topically applied Helicteres gardneriana extract used as anti-inflammatory agent.

Methods

Experiments were performed ex vivo in a well-controlled group of mice. The crude extract was obtained from leaves of the plant Helicteres gardneriana. Croton oil was applied into the ventral surface of the mouse’s right and left auricles in order to induce an inflammatory response. The left auricle was treated with crude extract, while the right one served as the control. After 6 h, the auricles were sectioned for measurements of edema intensity, myeloperoxidase activity and the formulation penetration rate.

Results

Croton oil induced inflammatory response in both auricles. The application of Helicteres gardneriana extract reduced significantly the edema of the auricle and inhibited the activity of the myeloperoxidase enzyme. The photoacoustic data showed that the propagation of the formulation was efficient to reach the deep region of the auricle, crossing the cartilage. The strong anti-inflammatory effect was associated with the observed deep penetration of the formulation.

Conclusion

This pre-clinical study showed the anti-inflammatory effect of Helicteres gardneriana extract. The photoacoustic technique was useful to demonstrate that this anti-inflammatory activity was associated with deep percutaneous penetration.

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ACKNOWLEDGMENTS

The authors are thankful to CAPES, CNPq and Fundação Araucária for the financial support of this work.

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Authors and Affiliations

  1. Departamento de Farmácia e Farmacologia, Universidade Estadual de Maringá, Avenida Colombo 5790, 87020-900, Maringá, Pr, Brazil

    Juliana Oliveira de Melo, Maria Conceição T. Truiti, Silmara Baroni & Ciomar A. Bersani-Amado

  2. Departamento de Física, Universidade Estadual de Maringá, Avenida Colombo 5790, 87020-900, Maringá, Pr, Brazil

    Franciana Pedrochi & Mauro L. Baesso

  3. Departamento de Ciências Morfológicas, Universidade Estadual de Maringá, Avenida Colombo 5790, 87020-900, Maringá, Pr, Brazil

    Luzmarina Hernandes

Authors
  1. Juliana Oliveira de Melo
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  2. Franciana Pedrochi
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  3. Mauro L. Baesso
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  4. Luzmarina Hernandes
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  5. Maria Conceição T. Truiti
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  6. Silmara Baroni
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  7. Ciomar A. Bersani-Amado
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Corresponding authors

Correspondence to Mauro L. Baesso or Ciomar A. Bersani-Amado.

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Oliveira de Melo, J., Pedrochi, F., Baesso, M.L. et al. Evidence of Deep Percutaneous Penetration Associated with Anti-Inflammatory Activity of Topically Applied Helicteres gardneriana Extract: A Photoacoustic Spectroscopy Study. Pharm Res 28, 331–336 (2011). https://doi.org/10.1007/s11095-010-0279-3

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  • DOI: https://doi.org/10.1007/s11095-010-0279-3

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