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Skin Localization of Cow’s Milk Proteins Delivered by a New Ready-to-Use Atopy Patch Test

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Purpose

Atopy patch tests (APTs) allow the detection of delayed allergies at the skin level. The localization of β-lactoglobulin delivered into the skin by an innovative ready-to-use APT (E-patch®) was investigated and the efficacy and safety of this device were assessed.

Methods

The E-patch® containing β-lactoglobulin was placed for 24 h in contact with hairless rat skin mounted in a Franz diffusion cell. Transdermal passage was monitored by measurement of β-lactoglobulin A-[methyl-14C] or by two-site enzyme immunoassay. An iterative skin stripping allowed measurement of the β-lactoglobulin penetrating the first external skin layers.

Results

After 24 h, 92% of β-lactoglobulin remained on the skin. The iterative skin strippings showed a 135-fold higher concentration of β-lactoglobulin in the stratum corneum than that found in the epidermis–dermis. Analysis of the solution in the receiver compartment by radioactivity assays or immunoassays indicates that intact protein did not cross the skin.

Conclusions

The E-patch® system allows native β-lactoglobulin to concentrate in the stratum corneum, in the vicinity of immunological cells, but does not lead to its systemic delivery. Therefore, it is suggested that this delivery system creates ideal conditions for promoting a positive topical response with reduced risk of systemic anaphylactic reactions caused by the native form of the β-lactoglobulin A.

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Acknowledgments

This work was supported by the CNRS, UPS, INRA-CEA, and by DBV Technologies (Boulogne Billancourt, France). The authors would like to thank Dr. Michel Renoir (UMR CNRS 8612) for help with electrophoretic analysis of β-lactoglobulin purity and Dr. Herve Bernard (INRA) for immunodosage. ANRT supported D. Soury (CIFRE).

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Authors and Affiliations

  1. Laboratoire de Physico-Chimie, Pharmacotechnie et Biopharmacie, UMR CNRS 8612, Faculté de Pharmacie, Université Paris Sud, Tour D5, 2ème étage 5 rue J.B. Clément, 92296, Châtenay-Malabry Cedex, France

    D. Soury, G. Barratt, P. Legrand, H. Chacun & G. Ponchel

  2. INRA-CEA, SPI, CE Saclay, Gif-sur-Yvette, France

    S. Ah-Leung

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  1. D. Soury
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  3. S. Ah-Leung
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  5. H. Chacun
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  6. G. Ponchel
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Correspondence to D. Soury.

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Soury, D., Barratt, G., Ah-Leung, S. et al. Skin Localization of Cow’s Milk Proteins Delivered by a New Ready-to-Use Atopy Patch Test. Pharm Res 22, 1530–1536 (2005). https://doi.org/10.1007/s11095-005-5881-4

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  • DOI: https://doi.org/10.1007/s11095-005-5881-4

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