The aim of this work was to evaluate the antioxidant capacity of some increasing polarity soluble fractions of hydroalcoholic extract of the leaves of Algerian Lamiaceae Calamintha baborensis Batt., including hexanoic (Hex), chloroformic (Chlor), ethyl acetate (EtOAc) and n-butanolic (n-BuOH) and four subfractions (A, B, C, D) resulting from fractionation on a polyamide column. The EtOAc and n-BuOH extracts of the plant gave the highest values of antioxidant power by different methods: ABTS, percentage inhibition of 68.9/81.7%; DPPH, 27.6/80.99%; ORAC/FRAP, 37.28/28.47 and 21.73/19.52 μM/mL, respectively; IC50 values, 23 and 53.5 ppm. In addition, they were evaluated for their total phenolic content. The antibacterial activity of extracts showed good results with hexanoic and chloroformic fractions against E. coli (19 mm and 19.2 mm diameter of inhibition zone and MIC values about 43 and 43.4 μg/mL, respectively.) Apolar fractions from the leaves of C. Baborensis Batt. were analyzed by gas chromatography–mass spectrometry (GC–MS). The main components of the essential oil of C baborensis Batt. are eugenol (27.04%) and 3-methoxy acetophenone (26.4%).
Similar content being viewed by others
References
W. F. Petrone, D. K. English, K. Wong, and J. M. McCord, Proc. Natl. Acad. Sci., 77, 1159 – 163 (1980).
B. Halliwell, C. E. Cross, and J. M. C. Gutteridge, J. Lab. Clin. Med., 119, 598 – 620 (1992).
T. Finkel and N. J. Holbrook, Nature, 408, 239 – 247 (2000).
S. E. Atawodi, Afr. J. Biotechnol., 4(2), 177 – 182 (2005).
I. S. R. Georgetti, R. Casagrande, V. M. Di Mambro, et al., Pharma Sci. Arlington, 5, 1 – 5 (2003).
M. P. Kahkonen, A. I. Hopia, H. J. Vuorela, et al., J. Agric. Food Chem., 47, 3954 – 3962 (1999).
Y. Velioglu, G. Mazza, L. Gao, and B. Oomah, J. Agric. Food Chem., 46, 4113 – 4117 (1998).
N. A. Botsoglou, S. H. Grigoropoulou, E. Botsoglou , and A. Govaris, Meat Sci., 65, 1193 – 1200 (2003).
V. Lagouri and D. Boskou, Food Sci., 37, 869 – 879 (1995).
G. Ruberto and M. T. Baratta, Food Chem., 69, 167 – 174 (2000).
N. Mimica-Dukic, B. Bozin, M. Sokovic, et al., Planta Med., 69, 413 – 419 (2003).
R. Phillips, N. Foy, Herbs, Pan Books, London (1990). ISBN 0-330-30725-8.
W. Brand-Williams, M. Cuvelier, and C. Berset, Food Sci. Technol., 28, 25 – 30 (1995).
C. Sanchez Moreno, J. A. Larrauri, and F. Saura-Calixto, J. Sci. Food Agric., 76(2), 270–276 (1998).
R. Pellegrini, N, Proteggente, A. Pannala, et al., Free Radical Biol. Med., 26, 1231 – 1237 (1999).
I. F. F. Benzie and J. Strain, Anal. Biochem., 239, 70 – 76 (1996).
P. Held, Performing oxygen radical absorbance capacity (ORAC) assays with SynergyTM HT multi-detection microplate reader. Biotek.http://www.biotek.com/resources/tech_res_detail.php(id=161)(2005). Accessed on 20 December 2013.
J. M. Andrew, J. Antimicrob. Chemother., 56, 60 – 76 (2005).
A. Chanwitheesuk, A. Teerawutgulrag, J. D. Kilburn, and N. Rakariyatham, Food Chem., 100, 1044 – 1048 (2007).
S. Ozturk and S. Ercisli, Food Control, 18, 535 – 540 (2007).
D. Dobravalskyté, P. R. Venskutonis, and T. Talou, Food Chem., 135, 1539 – 1546 (2012).
C. Souleles and N. Argyriadou, Planta Med., 56, 234 – 235 (1990).
A. P. Carnat, A. Chossegros, and J. L. Lamaison, J. Essent. Oil Res., 3, 361 – 362 (1991).
M. Kürkçüoglu, G. Iscan, T. Ozek, et al., J. Essent. Oil Res., 19, 285–287 (2007).
K. H. C. Baser and T. Ozek, Planta Med., 59, 390 (1993).
H. I. D. Dorman and R. Hiltunen, Food Chem., 88, 193 – 199 (2004).
N. Cotelle, J. L Bemier, J. P. Catteau, et al., Free Radical Biol. Med., 20, 35 – 43(1996).
I. I. Koleva, T. A. Van Beek, J. P. H. Linssen, et al., Phytochem. Anal., 13, 8 – 17 (2002).
N. Khled Khoudja, L. Boulekbache-Makhlouf, and K. Madani, Ind. Crops Prod., 52, 177 – 182 (2014).
D. Stagos, C. Spanou, N. Mossialos Portesis, et al., Food Chem. Toxicol., 50, 4115 – 4124 (2012).
R. Ksouri, W. Megdiche, H. Falleh, et al., C. R. Biol., 331, 865 – 873 (2008).
L. Panizzi, G. Flamini, P. L. Cioni, and I. Morelli, J. Ethnopharmacol., 39, 167 – 170.(1993).
D. Kitic, G. Stojanovic, R. Palic, and V. Randjelovic, J. Essent. Oil Res., 17, 701 – 703 (2005).
D. Kitic, R. Palic, G. Stojanovic, et al., J. Essent. Oil Res., 14, 150 (2002).
Acknowledgements
The authors wish to express their thanks to the Algerian Ministry of Higher Education and Research (MESRS) for financial support.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Seraoui, R., Benkiniouar, R., Akkal, S. et al. Phytochemical Investigation, Antioxidant and Antimicrobial Assays of Algerian Plant Calamintha baborensis Batt.. Pharm Chem J 52, 347–356 (2018). https://doi.org/10.1007/s11094-018-1820-7
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11094-018-1820-7