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MIUH Inhibits the Hippocampal Neuron Growth in Fetal Rat by Affecting the PTEN Pathway

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Abstract

Mild intrauterine hypoperfusion (MIUH) can induce placental dysfunction and lead to long-term changes during the process of brain development. A better understanding of the mechanism of MIUH will help in the development of new neuroprotective strategies for the placental chamber. To better understand the mechanism of the effect of MIUH on the neural development of offspring, we constructed a model of MIUH in pregnant rats. The proliferation, apoptosis, and autophagy of hippocampal neurons in fetal rats were studied via flow cytometry, immunofluorescence staining, JC-1 staining, western blotting, and real-time polymerase chain reaction at different time points (6, 24, 48, and 72 h). The results showed that MIUH significantly inhibited the proliferation of hippocampal neurons and promoted their apoptosis and autophagy. Simultaneously, MIUH could promote PTEN expression and affect the PTEN signaling pathway. bpV, an inhibitor of PTEN, could restore the inhibition of hippocampal nerve cell growth caused by MIUH. MIUH may inhibit neuronal proliferation and promote neuronal apoptosis and autophagy by regulating the PTEN signaling pathway.

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Data Availability

Datasets analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

This work was supported by National Key R&D Program of China (2018YFC1002902) to CL.

Funding

This work was supported by National Key R&D Program of China (2018YFC1002902) to CL.

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Authors and Affiliations

  1. Department of Gynaecology and Obstetrics, Shengjing Hospital of China Medical University, China Medical University, Shenyang, 110004, China

    Shaowei Yin, Yilin Meng & Caixia Liu

  2. Key Laboratory of Maternal-Fetal Medicine of Liaoning Province, Shenyang, 110004, China

    Shaowei Yin, Yilin Meng & Caixia Liu

  3. Department of Anesthesiology, Shengjing Hospital of China Medical University, China Medical University, No. 36, Sanhao Street, Heping District, Shenyang, 110004, Liaoning, China

    Yuan Wang

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  1. Shaowei Yin
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  2. Yilin Meng
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  3. Caixia Liu
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  4. Yuan Wang
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Contributions

SY, YW, and YM designed research; SY, YW, and YM performed research; SY, YW analyzed data; SY, CL wrote the paper, YW obtained funding, administrative, technical or material support, and supervision.

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Correspondence to Yuan Wang.

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The authors declare no conflict of interest.

Ethical Approval

The procedures followed were in accordance with Animal Ethics Committee of Shengjing Hospital of China (NO. 2018PS07K). The experimental procedure followed the United States National Institutes of Health Guide for the Care and Use of Laboratory Animals (NIH Publication No. 85-23, revised 1996).

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Yin, S., Meng, Y., Liu, C. et al. MIUH Inhibits the Hippocampal Neuron Growth in Fetal Rat by Affecting the PTEN Pathway. Neurochem Res 46, 2046–2055 (2021). https://doi.org/10.1007/s11064-021-03342-w

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  • DOI: https://doi.org/10.1007/s11064-021-03342-w

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