Abstract
G protein-coupled receptor 50 (GPR50) belongs to the G protein-coupled receptor which is highly homologous with the sequence of melatonin receptor MT1 and MT2. GPR50 expression has previously been reported in many brain regions, like cortex, midbrain, pons, amygdala. But, the distribution of GPR50 in the hippocampus and cortex and the cell types expressing GPR50 is not yet clear. In this study, we examined the distribution of GPR50 in adult male mice by immunofluorescence. Our results showed that GPR50 was localized in the CA1-3 pyramidal cells and the granule cells of the dentate gyrus. GPR50 was also expressed in excitatory and inhibitory neurons. As inhibitory neurons also contain many types, we found that GPR50 was localized in some interneurons in which it was co-expressed with the calcium-binding proteins calbindin, calretinin, and parvalbumin. Besides, similar results were seen in the cortex. The widespread expression of GPR50 in the hippocampus and cortex suggests that GPR50 may be associated with synaptic plasticity and cognitive function.
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Acknowledgements
This work was supported by grants from Scientific Study Project for Institutes of Higher Learning, Ministry of Education, Liaoning Province (LZ2019072, LZ2017001, L2016002) and Natural science foundation of Liaoning province of China (2015020672).
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SL, JY and XW contributed to the conception and design of the project. QL, BG, HS, NL and YZ contributed to the experiments. QL and YS analyzed and interpreted the data. JY, MN, and SL wrote the manuscript.
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Li, Q., Zhang, Y., Ge, BY. et al. GPR50 Distribution in the Mouse Cortex and Hippocampus. Neurochem Res 45, 2312–2323 (2020). https://doi.org/10.1007/s11064-020-03089-w
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DOI: https://doi.org/10.1007/s11064-020-03089-w