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Upregulated Expression of TRIM32 Is Involved in Schwann Cell Differentiation, Migration and Neurite Outgrowth After Sciatic Nerve Crush

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Abstract

Tripartite motif containing 32 (TRIM32), a member of the tripartite motif (TRIM) family, plays an indispensable role in myoblast proliferation. It also regulates neuron and skeletal muscle stem cell differentiation. Although it is of great importance, we know little about the roles of TRIM32 during peripheral nervous system injury. Here, we examined the dynamic changes of TRIM32 in acute sciatic nerve crush (SNC) model. After crush, TRIM32 rapidly increased and reached the climax at 1 week but then gradually declined to the normal level at 4 weeks post-injury. Meanwhile, we observed similar changes of Oct-6. What is more, we found co-localization of TRIM32 with S100 and Oct-6 in 1-week-injured tissues using double immunofluorescent staining. In further vitro experiments, enhancive expression of TRIM32 was detected during the process of cyclic adenosine monophosphate (cAMP)-induced Schwann cell differentiation and nerve growth factor (NGF)-induced PC12 cell neurite outgrowth. More interestingly, specific si-TRIM32-transfected RSC96 cells exhibited obvious reduction in the ability of migration. Taken together, we inferred that upregulated TRIM32 was not only involved in the differentiation and migration of Schwann cells but the neurite elongation after SNC.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 81471258, No. 31300902) and the Applied Basic Research Project of Nantong City (No. MS12015099).

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    Authors and Affiliations

    1. Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, 19 Qixiu Road, Nantong, Jiangsu, 226001, People’s Republic of China

      Yonghua Liu

    2. Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong University, Nantong, Jiangsu, 226001, People’s Republic of China

      Yonghua Liu, Weijie Wu, Chi Sun, Yuxi Liu, Zhaohui Yu & Youhua Wang

    3. Department of Pathogen Biology, Medical College, Nantong University, Nantong, Jiangsu, 226001, People’s Republic of China

      Yonghua Liu

    4. Department of Orthopaedics, Affiliated Jiangyin Hospital of Nantong University, Nantong University, Nantong, Jiangsu, 226001, People’s Republic of China

      Huiguang Yang & Zhengming Zhou

    5. Department of Orthopaedics, Affiliated Mental Health Center of Nantong University, Nantong University, Nantong, Jiangsu, 226001, People’s Republic of China

      Xiaojian Zhu

    6. Class 2, Grade 13, Clinical Medicine, Medical College, Nantong University, Nantong, Jiangsu, 226001, People’s Republic of China

      Yuyan Chen

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    1. Yonghua Liu
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    Yonghua Liu and Weijie Wu contributed equally to this work.

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    Liu, Y., Wu, W., Yang, H. et al. Upregulated Expression of TRIM32 Is Involved in Schwann Cell Differentiation, Migration and Neurite Outgrowth After Sciatic Nerve Crush. Neurochem Res 42, 1084–1095 (2017). https://doi.org/10.1007/s11064-016-2142-3

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