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Acrylamide Retards the Slow Axonal Transport of Neurofilaments in Rat Cultured Dorsal Root Ganglia Neurons and the Corresponding Mechanisms

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Abstract

Chronic acrylamide (ACR) exposure induces peripheral-central axonopathy in occupational workers and laboratory animals, but the underlying mechanisms remain unclear. In this study, we first investigated the effects of ACR on slow axonal transport of neurofilaments in cultured rat dorsal root ganglia (DRG) neurons through live-cell imaging approach. Then for the underlying mechanisms exploration, the protein level of neurofilament subunits, motor proteins kinesin and dynein, and dynamitin subunit of dynactin in DRG neurons were assessed by western blotting and the concentrations of ATP was detected using ATP Assay Kit. The results showed that ACR treatment results in a dose-dependent decrease of slow axonal transport of neurofilaments. Furthermore, ACR intoxication significantly increases the protein levels of the three neurofilament subunits (NF-L, NF-M, NF-H), kinesin, dynein, and dynamitin subunit of dynactin in DRG neurons. In addition, ATP level decreased significantly in ACR-treated DRG neurons. Our findings indicate that ACR exposure retards slow axonal transport of NF-M, and suggest that the increase of neurofilament cargoes, motor proteins, dynamitin of dynactin, and the inadequate ATP supply contribute to the ACR-induced retardation of slow axonal transport.

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Abbreviations

ACR:

Acrylamide

DRG:

Dorsal root ganglia

NF:

Neurofilament

NF-H:

Neurofilament heavy subunit

NF-M:

Neurofilament middle subunit

NF-L:

Neurofilament light subunit

ATP:

Adenosine triphosphate

KHC:

Kinesin heavy chain

NGF:

Nerve growth factor

IPP:

Image-pro plus

PBS:

Phosphate-buffered saline

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

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Acknowledgments

The authors gratefully acknowledge Dr. Anthony Brown (Ohio State University, Columbus, USA) for his generous gifts of pPA-GFP-NF-M plasmid and DsRed plasmid. The authors also thank Paula McKinney, Niraj Trivedi, Neal (Ohio State University) for technical assistance. This study was supported by the National Science Foundation of China (30872134).

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Authors and Affiliations

  1. Institute of Toxicology, School of Public Health, Shandong University, Jinan, 250012, China

    Lihong An, Cuili Zhang, Shuo Wang, Lulu Jiang & Keqin Xie

  2. Beijing Municipal Institute of Labour Protection, Taoranting Road, Xicheng District, Beijing, 100054, China

    Guozhen Li

  3. Institute of Environment and Health, School of Public Health, Shandong University, Jinan, 250012, China

    Lihong An & Jiliang Si

  4. College of Life Sciences, Shandong Normal University, Jinan, 250014, China

    Xiaoying Han

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  1. Lihong An
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Correspondence to Keqin Xie.

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An, L., Li, G., Si, J. et al. Acrylamide Retards the Slow Axonal Transport of Neurofilaments in Rat Cultured Dorsal Root Ganglia Neurons and the Corresponding Mechanisms. Neurochem Res 41, 1000–1009 (2016). https://doi.org/10.1007/s11064-015-1782-z

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  • DOI: https://doi.org/10.1007/s11064-015-1782-z

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