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Quercetin Promotes Neuronal and Behavioral Recovery by Suppressing Inflammatory Response and Apoptosis in a Rat Model of Intracerebral Hemorrhage

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Abstract

Intracerebral hemorrhage (ICH) is a common and devastating disease affecting millions of people worldwide annually. Exaggerated inflammation and apoptosis are two pivotal pathological processes for secondary brain injury after ICH. Quercetin, a flavonoid widely distributed in various herbs, fruits and vegetables, has been proved to improve neuronal functional recovery in spinal cord injury rats. However, the efficacy of quercetin in caring for post-ICH brain injury has not been investigated. In the present study, we established an ICH model by injecting type VII bacterial collagenase (0.5U) into the central striatum of male Sprague–Dawley rats. The animals were randomized to four groups: sham-operation group; ICH + vehicle group; ICH + 5 mg/kg quercetin group; and ICH + 50 mg/kg quercetin group. The expression levels of IL-1β, IL-4, IL-6 and TNF-α in the brain tissue were assayed by Real-time PCR, ELISA and Western Blot, and cell apoptosis was assayed by TUNEL and caspase-3 staining 3 days after model establishment. It was found that the lesion volume, the brain water content, the expression levels of the four inflammation markers and the number of apoptotic cells were reduced significantly in ICH rats receiving quercetin, especially in 50 mg/kg quercetin group. These results confirmed the therapeutic efficacy of quercetin in repairing brain injury, probably by inhibiting inflammatory response and apoptosis, thus promoting nerve functional restoration.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (BWS11J002, 81271391).

Ethical standards

All animal studies have been approved by the Animal Care and Use Committee of Shanghai Jiao Tong University and have therefore been performed in accordance with the ethical standards laid down in the 1964 Declaration of Helsinki and its later amendments.

Conflict of interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. Department of Plastic and Reconstructive Surgery, Shanghai 9th People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China

    Yifan Zhang

  2. Clinical College of General Hospital of Beijing Military Region, Anhui Medical University, Hefei, China

    Bo Yi & Yiwu Dai

  3. Affiliated Bayi Brain Hospital, General Hospital of Beijing Military Region, Beijing, China

    Jianhua Ma, Li Zhang, Hongtian Zhang & Yi Yang

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  1. Yifan Zhang
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  2. Bo Yi
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  4. Li Zhang
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  5. Hongtian Zhang
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  6. Yi Yang
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  7. Yiwu Dai
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Correspondence to Yi Yang or Yiwu Dai.

Additional information

Yifan Zhang and Bo Yi have contributed equally to this work.

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Zhang, Y., Yi, B., Ma, J. et al. Quercetin Promotes Neuronal and Behavioral Recovery by Suppressing Inflammatory Response and Apoptosis in a Rat Model of Intracerebral Hemorrhage. Neurochem Res 40, 195–203 (2015). https://doi.org/10.1007/s11064-014-1457-1

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  • DOI: https://doi.org/10.1007/s11064-014-1457-1

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