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Is There a Correlation Between In Vitro Antioxidant Potential and In Vivo Effect of Carvacryl Acetate Against Oxidative Stress in Mice Hippocampus?

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Abstract

This study investigated in vitro and in vivo antioxidant potential of carvacryl acetate (CA), a derivative of carvacrol, monoterpenic component of oregano. The correlation between in vitro and in vivo CA effects was also determined. In vitro tests measured thiobarbituric acid reactive species content, nitrite formation and hydroxyl radical levels. In vivo tests measured thiobarbituric acid reactive species content, nitrite concentration and reduced glutathione (GSH) levels, as well as glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase activities were measured, using mice hippocampus. The CA administrations for in vivo tests were intraperitoneally and acutely improved. CA reduced lipid peroxidation, nitrite and hydroxyl radical contents in vitro as well as lipid peroxidation and nitrite content in vivo. It also increased reduced GSH levels and GPx as well as catalase activities. Moreover, CA required a lower concentration to inhibit 50 % of free radicals measured in vitro than trolox. There was significant negative correlation between in vitro nitrite levels and in vivo reduced GSH levels; in vitro nitrite content and in vivo GPx activity as well as in vitro hydroxyl radical levels and in vivo SOD activity. To date, this is the first study which suggests vitro and in vivo antioxidant potential to this monoterpene and the correlation between these parameters.

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Acknowledgments

We would like to thank the National Council of Technological and Scientific Development (CNPq/Brazil) and the Research Supporting Foundation of State of Piaui (FAPEPI/Brazil) for the financial support and Stênio Gardel Maia for technical assistance.

Conflict of interest

The authors state no conflict of interest.

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Authors and Affiliations

  1. Postgraduate Program of Pharmacology, Federal University of Piauí, Teresina, PI, Brazil

    Lúcio Fernandes Pires & Rivelilson Mendes de Freitas

  2. Laboratory of Experimental Neurochemistry Research, Department of Biochemistry and Pharmacology, Center of Pharmaceutical Technology, Federal University of Piauí, Teresina, PI, Brazil

    Luciana Muratori Costa, Antonia Amanda Cardoso de Almeida, Oskar Almeida Silva & Rivelilson Mendes de Freitas

  3. Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Rua Coronel Nunes de Melo, Fortaleza, CE, 1127, Brazil

    Gilberto Santos Cerqueira

  4. Department of Pharmaceutical Science, Federal University of Paraíba, João Pessoa, PI, Brazil

    Damião Pergentino de Sousa

  5. Laboratório de Pesquisa em Neuroquímica Experimental, Curso de Farmácia, Universidade Federal do Piauí-UFPI, Campus Universitário Ministro Petrônio Portella, Bairro Ininga, Teresina, PI, CEP 64049-550, Brazil

    Lúcio Fernandes Pires & Rivelilson Mendes de Freitas

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  1. Lúcio Fernandes Pires
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Correspondence to Lúcio Fernandes Pires or Rivelilson Mendes de Freitas.

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Pires, L.F., Costa, L.M., de Almeida, A.A.C. et al. Is There a Correlation Between In Vitro Antioxidant Potential and In Vivo Effect of Carvacryl Acetate Against Oxidative Stress in Mice Hippocampus?. Neurochem Res 39, 758–769 (2014). https://doi.org/10.1007/s11064-014-1267-5

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