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Rutin from Dendropanax morbifera Leveille Protects Human Dopaminergic Cells Against Rotenone Induced Cell Injury Through Inhibiting JNK and p38 MAPK Signaling

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Abstract

Dendropanax morbifera Leveille (Araliaceae) is well known in Korean traditional medicine for a variety of diseases. Rotenone is a commonly used neurotoxin to produce in vivo and in vitro Parkinson’s disease models. This study was designed to elucidate the processes underlying neuroprotection of rutin, a bioflavonoid isolated from D. morbifera Leveille in cellular models of rotenone-induced toxicity. We found that rutin significantly decreased rotenone-induced generation of reactive oxygen species levels in SH-SY5Y cells. Rutin protected the increased level of intracellular Ca2+ and depleted level of mitochondrial membrane potential (ΔΨm) induced by rotenone. Furthermore, it prevented the decreased ratio of Bax/Bcl-2 caused by rotenone treatment. Additionally, rutin protected SH-SY5Y cells from rotenone-induced caspase-9 and caspase-3 activation and apoptotic cell death. We also observed that rutin repressed rotenone-induced c-Jun N-terminal kinase and p38 mitogen-activated protein kinase phosphorylation. These results suggest that rutin may have therapeutic potential for the treatment of neurodegenerative diseases associated with oxidative stress.

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Acknowledgments

This research was supported by Leaders in INdustry-university Cooperation (LINC) Project, Ministry of Education, Republic of Korea.

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Authors and Affiliations

  1. Department of Alternative Medicine, Gwangju University, Gwangju, 503-703, Republic of Korea

    Se-Eun Park, Ha-Na Oh & Seung Kim

  2. Central Department of Zoology, Tribhuvan University, Kirtipur, Kathmandu, Nepal

    Kumar Sapkota

  3. Department of Life Science and BK21-Plus Research Team for Bioactive Control Technology, Chosun University, Gwangju, 501-759, Republic of Korea

    Jun-Hui Choi & Sung-Jun Kim

  4. Department of Food Science and Biotechnology, Chonbuk National University, Iksan, 570-752, Republic of Korea

    Myung-Kon Kim & Young Hoi Kim

  5. SEROM Co., Jangheung-gun, 529-832, Republic of Korea

    Ki Man Kim

  6. Jangheung Research Institute for Mushroom Industry, Jangheung-gun, 529-851, Republic of Korea

    Kyung Je Kim

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  1. Se-Eun Park
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Correspondence to Sung-Jun Kim or Seung Kim.

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Se-Eun Park and Kumar Sapkota have contributed equally to this study.

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Park, SE., Sapkota, K., Choi, JH. et al. Rutin from Dendropanax morbifera Leveille Protects Human Dopaminergic Cells Against Rotenone Induced Cell Injury Through Inhibiting JNK and p38 MAPK Signaling. Neurochem Res 39, 707–718 (2014). https://doi.org/10.1007/s11064-014-1259-5

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  • DOI: https://doi.org/10.1007/s11064-014-1259-5

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