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Selenium Compounds Prevent Amyloid β-Peptide Neurotoxicity in Rat Primary Hippocampal Neurons

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Abstract

Neuropathological hallmarks of Alzheimer’s disease (AD) include amyloid plaque formation, neurofibrillary tangles, neuronal and synaptic loss. This study aims to identify the neuroprotective effects of the selenium compounds on the neurotoxicity of amyloid β(1–42) in primary cultures of murine hippocampal neurons. Samples were subjected to immunocytochemistry and western blotting techniques to determine the role of treatments on neuronal viability and synaptic protein SNAP-25. We observed a reduced cell viability amyloid β-peptide (1–42)-induced. When cells were co-treated with amyloid β-peptide (1–42) and selenium compounds, we verified a strong increase in relative cell viability and in the level of synaptic marker synaptosomal-associated protein SNAP-25 induced by selenium compounds.

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Acknowledgments

This work was supported by CNPq, FAPERGS and CAPES Brazil.

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No conflict declared.

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Authors and Affiliations

  1. Programa de Pós-Graduação em Saúde e Comportamento, Universidade Católica de Pelotas, Rua Gonçalves Chaves 373, sala 324, Pelotas, RS, CEP 96010-280, Brazil

    Gabriela Lorea Godoi, Janaína Fagundes Schulz, Fernanda Neutzling Kaufmann, Gabriele Ghisleni & Hiram Larangeira de Almeida Jr.

  2. Departamento de Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil

    Lisiane de Oliveira Porciúncula & Diogo Onofre Gomes de Souza

  3. Departamento de Bioquímica Toxicológica, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil

    João Batista da Rocha

Authors
  1. Gabriela Lorea Godoi
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  2. Lisiane de Oliveira Porciúncula
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  3. Janaína Fagundes Schulz
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  4. Fernanda Neutzling Kaufmann
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  5. João Batista da Rocha
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  6. Diogo Onofre Gomes de Souza
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  7. Gabriele Ghisleni
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  8. Hiram Larangeira de Almeida Jr.
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Corresponding author

Correspondence to Gabriele Ghisleni.

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Godoi, G.L., de Oliveira Porciúncula, L., Schulz, J.F. et al. Selenium Compounds Prevent Amyloid β-Peptide Neurotoxicity in Rat Primary Hippocampal Neurons. Neurochem Res 38, 2359–2363 (2013). https://doi.org/10.1007/s11064-013-1147-4

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  • DOI: https://doi.org/10.1007/s11064-013-1147-4

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