Abstract
Classical major histocompatibility complex (MHC) class I, first identified in the immune system, is also expressed in the developing and adult central nervous system (CNS). Although the MHC class I molecules have been found to be expressed in the CNS of different species, a necessary step to elucidate the temporal and spatial expression patterns of MHC class I molecules in the brain development has never been taken. Frozen sections were made from the brains of embryonic and postnatal C57BL/6 J mice, and the expression of H-2Db mRNA was examined by in situ hybridization. Immunofluorescence was also performed to define the cell types that express H2-Db in P15 mice. At E10.5, the earliest stage we examined, H2-Db was expressed in neuroepithelium of the brain vesicles. From E12.5 to P0, H2-Db expression was mainly located at cerebral cortex, neuroepithelium of the lateral ventricle, neuroepithelium of aquaeductus and developing cerebellum. From P4 to adult, H2-Db mRNA was detected at olfactory bulb, hippocampus, cerebellum and some nerve nuclei. The major cell types expressing H-2Db in P15 hippocampus, cerebral cortex and olfactory bulb were neuron. H2-Kb signal paralleled that of H2-Db and the expression levels of the two molecules were comparable throughout the brain. The investigation of the expression pattern of H-2Db at both embryonic and postnatal stages is important for further understanding the physiological and pathological roles of H2-Db in the developing CNS.
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This work was supported by National Nature Science Foundation of China (31070807, 31100649) and Research Fund for the Doctoral Program of Higher Education of China (20100092110040).
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Liu, J., Shen, Y., Li, M. et al. The Expression Pattern of Classical MHC Class I Molecules in the Development of Mouse Central Nervous System. Neurochem Res 38, 290–299 (2013). https://doi.org/10.1007/s11064-012-0920-0
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DOI: https://doi.org/10.1007/s11064-012-0920-0