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High-Glucose and S100B Stimulate Glutamate Uptake in C6 Glioma Cells

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Abstract

Diabetes mellitus is a disease associated with several changes in the central nervous system, including oxidative stress and abnormal glutamatergic neurotransmission, and the astrocytes play an essential role in these alterations. In vitro studies of astroglial function have been performed using cultures of primary astrocytes or C6 glioma cells. Herein, we investigated glutamate uptake, glutamine synthetase and S100B secretion in C6 glioma cells cultured in a high-glucose environment, as well as some parameters of oxidative stress and damage. C6 glioma cells, cultured in 12 mM glucose medium, exhibited signals of oxidative and nitrosative stress similar to those found in diabetes mellitus and other models of diabetic disease (decrease in glutathione, elevated NO, DNA damage). Interestingly, we found an increase in glutamate uptake and S100B secretion, and a decrease in glutamine synthetase, which might be linked to the altered glutamatergic communication in diabetes mellitus. Moreover, glutamate uptake in C6 glioma cells, like primary astrocytes, was stimulated by extracellular S100B. Aminoguanidine partially prevented the glial alterations induced by the 12 mM glucose medium. Together, these data emphasize the relevance of astroglia in diabetes mellitus, as well as the importance of glial parameters in the evaluation of diabetic disease progression and treatment.

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Acknowledgments

This work was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), FINEP (IBN 01.06.0842-00) and INCT-National Institute of Science and Technology for Excitotoxicity and Neuroprotection.

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  1. Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Ramiro Barcelos, 2600-anexo, Porto Alegre, RS, 90035-003, Brazil

    Ana Carolina Tramontina, Patrícia Nardin, André Quincozes-Santos, Lucas Tortorelli, Krista Minéia Wartchow, Ana Cristina Andreazza, Elizandra Braganhol, Diogo Onofre Gomes de Souza & Carlos-Alberto Gonçalves

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  1. Ana Carolina Tramontina
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  2. Patrícia Nardin
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Correspondence to Carlos-Alberto Gonçalves.

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Tramontina, A.C., Nardin, P., Quincozes-Santos, A. et al. High-Glucose and S100B Stimulate Glutamate Uptake in C6 Glioma Cells. Neurochem Res 37, 1399–1408 (2012). https://doi.org/10.1007/s11064-012-0722-4

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  • DOI: https://doi.org/10.1007/s11064-012-0722-4

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