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Oxidative Stress, Cytokine/Chemokine and Disruption of Blood–Brain Barrier in Neonate Rats After Meningitis by Streptococcus agalactiae

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Abstract

We verify the levels of cytokine/chemokine, myeloperoxidase activity, oxidative stress and disruption of BBB in hippocampus and cortex of the neonate Wistar rats after meningitis by S. agalactiae. In the hippocampus the levels were increased of CINC-1 at 6 h and 12 h, IL-1β at 6, 12 and 24 h, IL-6 at 6, 24 and 96 h, IL-10 at 24, 48 and 96 h and TNF-α at 24 h and 96 h. In the cortex the CINC-1 and IL-1β levels were found increased at 6 h. The MPO activity was significantly elevated at 24, 48 and 98 h in hippocampus and at 6, 12, 24, 48 and 96 h in the cortex. The breakdown of BBB started at 12 h.TBARS levels were elevated in the hippocampus at 6, 12, 24, 48, 72 and 96 h and cortex at 72 and 96 h. Protein carbonyls were elevated in the hippocampus and cortex at 6, 24, 48, 72 and 96 h. There was a decrease of SOD activity in hippocampus and in cortex. Catalase activity was elevated in hippocampus at 6 h and in the cortex at 12 and 96 h. Neonatal bacterial infections of the CNS are severe, the interference with the complex network of cytokines/chemokine, other inflammatory mediators and oxidants tend to aggravate the illness and can be involved in the breakdown of the BBB.

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Abbreviations

BBB:

Blood-brain barrier

CAT:

Calatase

CFU:

Colony-forming unit

CINC-1:

Cytokine-Induced neutrophil chemoattractant

CSF:

Cerebrospinal fluid

DNA:

Deoxyribonucleic acid

DNPH:

Dinitrophenylhidrazine

GBS:

Group B Streptococcus

IL-6:

Interleukin 6

IL-1β:

Interleukin 1 betha

IL-10:

Interleukin 10

ip:

Intra peritoneal

MPO:

Myeloperoxidase activity

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

TBARS:

Thiobarbituric acid reactive species

TNF-α:

Tumor necrosis factor alpha

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Acknowledgments

This research was supported by grants from CNPq, FAPEMIG, FAPESC, UNESC and Instituto Nacional de Ciência e Tecnologia Translacional em Medicina (INCT-TM).

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Authors and Affiliations

  1. Laboratório de Microbiologia Experimental and Instituto Nacional de Ciência e Tecnologia Translacional em Medicina, Programa de Pós-Graduação em Ciências da Saúde, Unidade Acadêmica de Ciências da Saúde, Universidade do Extremo Sul Catarinense, Criciúma, SC, Brazil

    Tatiana Barichello, Joelson C. Lemos, Jaqueline S. Generoso, Andreza L. Cipriano, Graziele L. Milioli & Danielle M. Marcelino

  2. Laboratório de Fisiopatologia and Instituto Nacional de Ciência e Tecnologia Translacional em Medicina, Programa de Pós-Graduação em Ciências da Saúde, Unidade Acadêmica de Ciências da Saúde, Universidade do Extremo Sul Catarinense, Criciúma, SC, Brazil

    Francieli Vuolo & Felipe Dal-Pizzol

  3. Programa de Pós-Graduação em Ciências da Saúde, Universidade do Sul Catarinense, Tubarão, SC, Brazil

    Fabricia Petronilho

  4. Laboratório de Imunofarmacologia, Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil

    Márcia Carvalho Vilela & Antonio Lucio Teixeira

  5. Departamento de Biologia Animal, Universidade Federal de Viçosa, Viçosa, MG, Brazil

    Márcia Carvalho Vilela

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  1. Tatiana Barichello
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  2. Joelson C. Lemos
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  3. Jaqueline S. Generoso
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Correspondence to Tatiana Barichello.

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Barichello, T., Lemos, J.C., Generoso, J.S. et al. Oxidative Stress, Cytokine/Chemokine and Disruption of Blood–Brain Barrier in Neonate Rats After Meningitis by Streptococcus agalactiae . Neurochem Res 36, 1922–1930 (2011). https://doi.org/10.1007/s11064-011-0514-2

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  • DOI: https://doi.org/10.1007/s11064-011-0514-2

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