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Interleukin-1β Inhibits Voltage-Gated Sodium Currents in a Time- and Dose-Dependent Manner in Cortical Neurons

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Abstract

Interleukin-1β (IL-1β) is a multifunctional proinflammatory cytokine that plays a key role in the injuries and diseases of the central nervous system (CNS). A voltage-gated Na+ channel is essential for the excitability and electrical properties of neurons. However, it is not known whether IL-1β directly affects the central Na+ channels. In the present study, we examined the effects of IL-1β on Na+ currents in cultured cortical neurons using patch-clamp recording. Our results showed that IL-1β suppressed Na+ currents through its receptor in a time- and dose-dependent manner, but did not alter the voltage-dependent activation and inactivation. PKC and then p38 MAPK were involved in this inhibition. The spike amplitude was also inhibited by IL-1β in the doses that decreased the Na+ currents. Our findings revealed the inhibition of chronic IL-1β treatment on voltage-gated Na+ channels in the CNS, and showed that the action potential (AP) amplitude was reduced by IL-1β due to a decrease of Na+ currents.

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Acknowledgments

This work was supported by National Key Basis Research Program of Ministry of Science and Technology of China (973 grant number: 2006CB504105 and 2009CB941301) and National Natural Science Foundation of China (grant number: 30670500 and 30871287).

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Authors and Affiliations

  1. College of Life Sciences, Hebei University, 180 Wusidong Rd, Baoding, Hebei, 071002, People’s Republic of China

    Chen Zhou, Cui Qi, Fei Wang, Weiwei Zhang, Junzhan Jing & Xianjiang Kang

  2. State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, 5 Yiheyuan Rd, Beijing, 100871, People’s Republic of China

    Juanjuan Zhao, Chen Li & Zhen Chai

Authors
  1. Chen Zhou
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  2. Cui Qi
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  3. Juanjuan Zhao
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  4. Fei Wang
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  5. Weiwei Zhang
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  6. Chen Li
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  7. Junzhan Jing
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  8. Xianjiang Kang
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  9. Zhen Chai
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Correspondence to Chen Zhou or Zhen Chai.

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Chen Zhou and Cui Qi contributed equally to this work.

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Zhou, C., Qi, C., Zhao, J. et al. Interleukin-1β Inhibits Voltage-Gated Sodium Currents in a Time- and Dose-Dependent Manner in Cortical Neurons. Neurochem Res 36, 1116–1123 (2011). https://doi.org/10.1007/s11064-011-0456-8

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