Abstract
Stress leads to changes in homeostasis and internal balance of the body and is known to be one of important factors in the development of several diseases. In the present study, we investigated changes in glucocorticoid receptor (GR) and ionized calcium-binding adapter molecule 1 (Iba-1) immunoreactivity in the adult and aged gerbil hippocampus after chronic restraint stress. Serum corticosterone level was much higher in both the stress-groups than the control groups. No neuronal death was found in all hippocampal subregions of the adult and aged gerbil after chronic restraint stress. GR immunoreactivity was decreased in both the adult and aged groups after repeated restraint stress; however, GR immunoreactivity in the adult-stress-group was decreased much more than that in the aged-stress-group. Iba-1 immunoreactive microglia were hypertrophied and activated in the adult group after repeated restraint stress; in the aged-stress-group, there was no any significant change in Iba-1 immunoreactive microglia. In brief, level of GR, not Iba-1, in the hippocampus was much decreased in the adult gerbil compared to the aged gerbil following chronic restraint stress.
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Acknowledgments
The authors would like to thank Mr. Seung Uk Lee and Ms. Hyun Sook Kim for their technical help. This study was supported by the Regional Core Research Program funded by the Korea Ministry of Education, Science and Technology (Medical and Bio-material Research Center) and by a grant (2010K000823) from Brain Research Center of the twenty-firstst Century Frontier Research Program funded by the Ministry of Education, Science and Technology, the Republic of Korea.
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Joon Ha Park and Ki-Yeon Yoo contributed equally to this article.
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Park, J.H., Yoo, KY., Lee, C.H. et al. Comparison of Glucocorticoid Receptor and Ionized Calcium-Binding Adapter Molecule 1 Immunoreactivity in the Adult and Aged Gerbil Hippocampus Following Repeated Restraint Stress. Neurochem Res 36, 1037–1045 (2011). https://doi.org/10.1007/s11064-011-0444-z
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DOI: https://doi.org/10.1007/s11064-011-0444-z