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Neural Markers Espression in Rat Bone Marrow Mesenchymal Stem Cell Cultures Treated with Neurosteroids

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Abstract

The aim of the present investigation is to study the effects of DEX or E2 treatment during differentiation towards neural cell line of rat BM-MSCs in culture. In order to better characterize biochemically our in vitro model, we evaluate by western blotting and immunocytochemical analysis some neural lineage markers (nestin, neurofilament, β-tubulin) and MAP-Kinases. An enhanced expression of the neural markers and MAP-Kinase in DEX-treated BM-MSCs cultures is found. In addition, E2-treatment increases MAP-Kinase and β-tubulin expression, but it decreases nestin and neurofilament expression. In conclusion, our findings highlight a significant up and down modulation of nestin, neurofilament, β-tubulin and MAP-Kinases expression in neurosteroids-treated BM-MSCs. In particular, our results clarify the molecular mechanism involved during eventual differentiation of these stem cells treated with DEX and E2, addressed towards a neural cell line, that may express neurotrophic receptors and release neurotrophines particularly implicated during neurogenesis processes.

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Acknowledgments

The authors particularly acknowledged Prof. Abel Lajtha, Editor in Chief of this prestigious International journal, excellent scientific guide, who contributed greatly to stimulate the development and advancement of international neurochemical research. In addition, the authors are very grateful to Prof. Anna Maria Giuffrida Stella, mentor of Prof. Roberto Avola, for excellent scientific suggestions and advices given during the preparation of this manuscript.

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Authors and Affiliations

  1. Department of Physiological Sciences, University of Catania, Catania, Italy

    Daniela Bronzi, Floriana Laureanti & Guido Li Volsi

  2. Department of Chemical Sciences, Section of Biochemistry and Molecular Biology, University of Catania, Viale A. Doria 6, 95125, Catania, Italy

    Vincenzo Bramanti, Daniele Tomassoni, Sonia Grasso & Roberto Avola

Authors
  1. Daniela Bronzi
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  2. Vincenzo Bramanti
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  3. Daniele Tomassoni
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  4. Floriana Laureanti
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  5. Sonia Grasso
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  6. Guido Li Volsi
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  7. Roberto Avola
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Corresponding author

Correspondence to Roberto Avola.

Additional information

D. Bronzi and V. Bramanti contributed equally.

Special Issue: In Honor of Dr. Abel Lajtha.

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Bronzi, D., Bramanti, V., Tomassoni, D. et al. Neural Markers Espression in Rat Bone Marrow Mesenchymal Stem Cell Cultures Treated with Neurosteroids. Neurochem Res 35, 2154–2160 (2010). https://doi.org/10.1007/s11064-010-0283-3

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  • DOI: https://doi.org/10.1007/s11064-010-0283-3

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