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Oroxylin A, a Flavonoid, Stimulates Adult Neurogenesis in the Hippocampal Dentate Gyrus Region of Mice

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Abstract

Previously, we reported the cognitive enhancing effects of oroxylin A in unimpaired mice and its memory ameliorating activity in various memory impaired mice. To elucidate the mechanism mediating the cognitive effects of oroxylin A, this study examined the consequences of oroxylin A administration on neurogenesis in the hippocampal dentate gyrus using immunostaining for 5-bromo-2-deoxyuridine (BrdU) incorporation. In addition, we determined whether the new cells adopted a neuronal or glial fate by examining the co-localization of BrdU staining with neuronal or glial markers. Administration of oroxylin A in a dose-dependent and time-dependent manner increased the number of BrdU-incorporating cells. Moreover, the percentage of BrdU-incorporating cells co-localized with neuronal markers, neuronal nuclei, was significantly increased by the oroxylin A administration. These results suggest that the increased neurogenesis induced by the administration of oroxylin A could be, at least in part, associated with its positive effects on cognitive processing.

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Acknowledgments

This research was supported by a grant funded by Korean Food and Drug Administration (2005; L-05-03-2-CHM-628-B).

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Authors and Affiliations

  1. Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Hoeki-dong, Dongdaemoon-Ku, Seoul, 130-701, Republic of Korea

    Seungjoo Lee, Dong Hyun Kim, Chang Hwan Lee & Jong Hoon Ryu

  2. Department of Oriental Pharmaceutical Science, Kyung Hee University, Hoeki-dong, Dongdaemoon-Ku, Seoul, 130-701, Republic of Korea

    Dong Hyun Kim, Chang Hwan Lee & Jong Hoon Ryu

  3. Kyung Hee East–West Pharmaceutical Research Institute, College of Pharmacy, Kyung Hee University, Hoeki-dong, Dongdaemoon-Ku, Seoul, 130-701, Republic of Korea

    Jong Hoon Ryu

  4. Department of Herbal Medicinal Pharmacology, College of Herbal Bio-industry, Daegu Haany University, Gyeongsan, 712-715, Republic of Korea

    Seungjoo Lee & Ji Wook Jung

  5. Department of Food and Nutrition, Andong National University, Andong, 760-749, Republic of Korea

    Dong Hwa Lee, Su Jin Jeon & Kun Ho Son

  6. Department of Pharmacology, School of Medicine and Center for Geriatric Neuroscience Research, IBST, Konkuk University, Seoul, Republic of Korea

    Chan Young Shin

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  1. Seungjoo Lee
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  2. Dong Hyun Kim
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  7. Ji Wook Jung
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Correspondence to Jong Hoon Ryu.

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Present name: The name of Seungjoo Lee was legally changed into Seungheon Lee.

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Lee, S., Kim, D.H., Lee, D.H. et al. Oroxylin A, a Flavonoid, Stimulates Adult Neurogenesis in the Hippocampal Dentate Gyrus Region of Mice. Neurochem Res 35, 1725–1732 (2010). https://doi.org/10.1007/s11064-010-0235-y

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  • DOI: https://doi.org/10.1007/s11064-010-0235-y

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