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Effect of NRG1, GDNF, EGF and NGF in the Migration of a Schwann Cell Precursor Line

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An Erratum to this article was published on 10 April 2014

Abstract

The Schwann cells are the myelinating glia of the peripheral nervous system that originated during development from the highly motile neural crest. However, we do not know what the guidance signals for the Schwann cell precursors are. Therefore, we set to test some of the known neurotrophins that are expressed early in developing embryos and have been shown to be critical for the survival and patterning of developing glia and neurons. The goal of this study was to determine more specifically if GDNF, NRG1 and NGF are chemoattractants and/or chemokinetic molecules for a Schwann cell precursor line, the Spl201. We performed live chemoattraction assays, with imaging and also presented these molecules as part of their growing substrate. Our results show for the first time that GDNF and NRG1 are potent chemoattractive and chemokinetic molecules for these cells while NGF is a chemokinetic molecule stimulating their motility.

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Acknowledgments

We thank Sonsoles de Lacalle for help writing this manuscript. This work was partly supported by a NIH-MBRS SCORE-5S06GM048680-13 to MEdB.

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Authors and Affiliations

  1. Department of Biology, California State University Northridge, MC 8303.18111 Nordhoff Street, Northridge, CA, 91330, USA

    Martha Cornejo, Deborah Nambi, Christopher Walheim, Matthew Somerville, Jacquae Walker, Lino Kim, Lauren Ollison, Graciel Diamante & Maria Elena de Bellard

  2. Division of Biology, California Institute of Technology, 139-74, Pasadena, CA, 91125, USA

    Saurabh Vyawahare

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  1. Martha Cornejo
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Correspondence to Maria Elena de Bellard.

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Martha Cornejo, Deborah Nambi and Christopher Walheim these authors contributed equally.

An erratum to this article is available at http://dx.doi.org/10.1007/s11064-014-1279-1.

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Cornejo, M., Nambi, D., Walheim, C. et al. Effect of NRG1, GDNF, EGF and NGF in the Migration of a Schwann Cell Precursor Line. Neurochem Res 35, 1643–1651 (2010). https://doi.org/10.1007/s11064-010-0225-0

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