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Comparison of Newly Generated Doublecortin-immunoreactive Neuronal Progenitors in the Main Olfactory Bulb among Variously Aged Gerbils

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Abstract

In the present study, we investigated age-related differences in neuronal progenitors in the gerbil main olfactory bulb (MOB) using doublecortin (DCX), a marker for neuronal progenitors which differentiate into neurons in the brain. No difference in the number of neuronal nuclei (NeuN)-immunoreactive neurons was found in the MOB at variously aged gerbils. At postnatal month (PM) 1, DCX immunoreaction was detected in all layers of the MOB except for the olfactory nerve layer. At this time point, DCX-immunoreactive cells (neuronal progenitors) were very abundant; however, they did not have fully developed-processes. From PM 3, the number of DCX-immunoreactive neuronal progenitors was decreased with age. At PM 6, DCX-immunoreactive cells showed very well-developed processes. In western blot analysis, DCX protein level in the MOB was highest at PM 1. Thereafter, levels of DCX protein were decreased with age. In the subventricular zone of the lateral ventricle, the number of Ki-67-immunoractive cells (proliferating cells) was also significantly decreased with age. In addition, increases of α-synuclein-immunoreactive structures were observed in the MOB with age. These results suggest that decrease in DCX-immunoreactive neuronal progenitors and its protein levels in the MOB with age may be associated with reduction of cell proliferation in the SVZ and with an increase in α-synuclein in the MOB.

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Acknowledgments

The authors would like to thank Mr. Seok Han, Mr. Seung Uk Lee and Ms. Hyun Sook Kim for their technical help and Seung-Hae Kwon of the Korean Basic Science Institute Chuncheon Center for technical assistance with the confocal image analyses in this study. This research was supported by a grant (2009K001290) from Brain Research Center of the twenty-first Century Frontier Research Program funded by the Ministry of Education, Science and Technology, the Republic of Korea, by the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2009-0084089), and by a grant from Hallym University Medical Center Research Fund (01-2007-20).

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Authors and Affiliations

  1. Department of Anatomy and Neurobiology, and Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University, Chuncheon, 200-702, South Korea

    Jung Hoon Choi, Ki-Yeon Yoo, Choong Hyun Lee, Ok Kyu Park, Bing Chun Yan, Hua Li & Moo-Ho Won

  2. Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, South Korea

    In Koo Hwang

  3. Division of Applied Chemistry and Biotechnology, Hanbat National University, Daejeon, 305-719, South Korea

    Jeong Ho Park

  4. Department of Pediatrics, Kangnam Sacred Heart Hospital, College of Medicine, Hallym University, Seoul, 150-950, South Korea

    Sung Koo Kim

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  1. Jung Hoon Choi
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Correspondence to Sung Koo Kim or Moo-Ho Won.

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Jung Hoon Choi and Ki-Yeon Yoo contributed equally to this article.

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Choi, J.H., Yoo, KY., Lee, C.H. et al. Comparison of Newly Generated Doublecortin-immunoreactive Neuronal Progenitors in the Main Olfactory Bulb among Variously Aged Gerbils. Neurochem Res 35, 1599–1608 (2010). https://doi.org/10.1007/s11064-010-0220-5

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