Abstract
Neuroglycan C (NGC) is a transmembrane-type chondroitin sulfate proteoglycan that promotes neurite outgrowth. To identify the ligand of NGC, we applied a detergent-solubilized membrane fraction of fetal rat brains to an NGC-immobilized affinity column. Several proteins were eluted from the column including an 18 kDa-band protein recognized by an anti-pleiotrophin antibody. The binding of pleiotrophin (PTN) to NGC was confirmed by a quartz crystal microbalance method and had a Kd of 8.7 nM. PTN bound to the acidic amino acid cluster of the NGC extracellular domain. In addition, PTN bound to both chondroitin sulfate-bearing NGC and chondroitinase-treated NGC prepared from the neonatal rat brain. These results suggest that NGC interacts with PTN.
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Acknowledgments
We thank to Dr. M. Watanabe (Aichi Human Service Center) for his helpful discussions. The present study was supported in part by Grants-in-aid for Scientific Research from the Ministry of Education, Science, Culture and Sports of Japan, and from the Japan Society for the Promotion of Science.
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Nakanishi, K., Tokita, Y., Aono, S. et al. Neuroglycan C, A Brain-Specific Chondroitin Sulfate Proteoglycan, Interacts with Pleiotrophin, A Heparin-Binding Growth Factor. Neurochem Res 35, 1131–1137 (2010). https://doi.org/10.1007/s11064-010-0164-9
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DOI: https://doi.org/10.1007/s11064-010-0164-9