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Danthron Induces DNA Damage and Inhibits DNA Repair Gene Expressions in GBM 8401 Human Brain Glioblastoma Multiforms Cells

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Abstract

Our primary studies had shown that danthron induced cytotoxic effects, including apoptosis and inhibition of migration and invasion. However, danthron-affected DNA damage and repair gene expressions are not clear. In this study, we investigated to examine whether or not danthron induced DNA damage and inhibited DNA repair gene expression in human brain glioblastoma multiforms (GBM 8401) cells. The results from Comet assay indicated that incubation of GBM 8401 cells with 0, 50, 100 and 150 μM of danthron led to a longer DNA migration smear based on the single cell electrophoresis (Comet tail). The results from real-time PCR assay demonstrated that 100 μM of danthron for 24 h treatment in GBM 8401 cells led to decrease all examined ataxia telangiectasia mutated (ATM), ataxia-telangiectasia and Rad3-related (ATR), breast cancer 1, early onset (BRCA-1), 14-3-3 proteins sigma (14-3-3σ), DNA-dependent serine/threonine protein kinase (DNA-PK) and O 6-methylguanine-DNA methyltransferase (MGMT) mRNA expressions. Taken together, the present study showed that danthron caused DNA damage and inhibited DNA repair genes, which may be the factors for danthron-inhibited cell growth in vitro.

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Acknowledgments

This work was supported by grant CMU97-037 from China Medical University, Taichung, Taiwan.

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Authors and Affiliations

  1. Department of Clinical Pathology, Cheng Hsin General Hospital, Taipei, Taiwan

    Hsu-Feng Lu

  2. College of Human Ecology, Fu-Jen Catholic University, Taipei, Taiwan

    Hsu-Feng Lu

  3. School of Post-Baccalaureate Chinese Medicine, China Medical University, Taichung, Taiwan

    Tung-Yuan Lai

  4. Department of Chinese Internal Medicine, China Medical University Hospital, Taichung, Taiwan

    Tung-Yuan Lai

  5. Department of Internal Medicine, China Medical University Hospital, Taichung, Taiwan

    Te-Chung Hsia

  6. School of Chinese Medicine, China Medical University, Taichung, Taiwan

    Te-Chung Hsia

  7. Department of Family Medicine, Taichung Veterans General Hospital, Taichung, Taiwan

    Yih-Jing Tang

  8. Department of Pharmacology, China Medical University, Taichung, Taiwan

    Jai-Sing Yang

  9. Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan

    Jo-Hua Chiang & Chi-Cheng Lu

  10. Department of Research and Education, Cheng Hsin General Hospital, Taipei, Taiwan

    Chi-Ming Liu

  11. Research Institute of Medical Laboratory Science and Biotechnology, Yuanpei University, Hsinchu, Taiwan

    Hai-Lung Wang

  12. Department of Biological Science and Technology, China Medical University, No 91, Hsueh-Shih Road, Taichung, 404, Taiwan

    Jing-Gung Chung

  13. Department of Biotechnology, Asia University, Wufeng, Taichung, Taiwan

    Jing-Gung Chung

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  1. Hsu-Feng Lu
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  2. Tung-Yuan Lai
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  3. Te-Chung Hsia
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  4. Yih-Jing Tang
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  5. Jai-Sing Yang
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  7. Chi-Cheng Lu
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  8. Chi-Ming Liu
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  9. Hai-Lung Wang
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  10. Jing-Gung Chung
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Correspondence to Jing-Gung Chung.

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Lu, HF., Lai, TY., Hsia, TC. et al. Danthron Induces DNA Damage and Inhibits DNA Repair Gene Expressions in GBM 8401 Human Brain Glioblastoma Multiforms Cells. Neurochem Res 35, 1105–1110 (2010). https://doi.org/10.1007/s11064-010-0161-z

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  • DOI: https://doi.org/10.1007/s11064-010-0161-z

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