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Transient Receptor Potential Vanilloid-1 (TRPV1) and Ankyrin-1 (TRPA1) Participate in Visceral Hyperalgesia in Chronic Water Avoidance Stress Rat Model

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Abstract

Stressfull life events have powerful influences on visceral perception of certain IBS patients. In the present study, we aimed to examine the involvement of TRPV1 and TRPA1 in the stress-induced visceral hyperalgesia. Rats were exposed to 1-h water avoidance stress (WAS) daily for 10 consecutive days. The abdominal withdrawal reflex (AWR) to colorectal distension was assessed at the end of the 10-day period. Western-blotting analysis was applied to investigate the alterations of TRPV1 and TRPA1 in the colonic afferent dorsal root ganglia (DRG). Compared with control rats, the WAS-treated rats demonstrated a significant increase in the AWR with the pressure ≥40 mm Hg (P < 0.05). Meanwhile, in the WAS-treated rats, western-blotting analysis showed significant upregulation of TRPV1 and TRPA1 in the colonic afferent DRG. The results indicate that WAS could induce the upregulation of TRPV1 and TRPA1 in the colonic afferent DRG, and both receptors may be candidate molecules involved in the stress-induced visceral hyperalgesia in rats.

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Acknowledgments

This work is supported by the National Natural Science Foundation of China (NSFC, 30770990) and the Department of Science and Technology of Shandong Province of China (Y2005C02). The authors appreciate the considerable assistance from the Key Laboratory of Cardiovascular Remodeling and Function Research in Qilu Hospital of Shandong University.

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Authors and Affiliations

  1. Department of Gastroenterology, Qilu Hospital, Shandong University, 250012, Jinan, People’s Republic of China

    Yan-Bo Yu, Jing Yang, Xiu-Li Zuo, Li-Jun Gao, Peng Wang & Yan-Qing Li

  2. The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health, Qilu Hospital, Shandong University, 250012, Jinan, People’s Republic of China

    Yan-Bo Yu

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  1. Yan-Bo Yu
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  2. Jing Yang
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Correspondence to Yan-Qing Li.

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Yu, YB., Yang, J., Zuo, XL. et al. Transient Receptor Potential Vanilloid-1 (TRPV1) and Ankyrin-1 (TRPA1) Participate in Visceral Hyperalgesia in Chronic Water Avoidance Stress Rat Model. Neurochem Res 35, 797–803 (2010). https://doi.org/10.1007/s11064-010-0137-z

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