Skip to main content
SpringerLink
Log in

The Expression Pattern of Heterogeneous Nuclear Ribonucleoprotein R in Rat Retina

  • ORIGINAL PAPER
  • Published:
Neurochemical Research Aims and scope Submit manuscript

Abstract

The heterogeneous nuclear ribonucleoproteins (hnRNPs) play important roles in DNA repairing, cell signaling, telomere biogenesis, and in regulating gene expression at both transcriptional and translational levels. In the present study, we demonstrated that the expression of hnRNP-R1 and hnRNP-R2 is developmentally regulated in rat retina. The neural specific isoform hnRNP-R2 is expressed in 7-day postnatal rat retina, but not in the adult retina. The positive immunohistochemistry signal of hnRNP-R1 is extensively distributed in the outer plexiform layer, inner nuclear layer and ganglion cell layer of rat retina. Double staining experiments showed that the positive signal of hnRNP-R1 is distributed in ON-type bipolar cells and localized in the cytoplasm, dendrites and axon terminals. In addition, the hnRNP-R1 distribution is regulated in rat retina during circadian. The present investigation suggests that hnRNP-R may play roles in retinal development and light-elicited cellular activities.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Pinol-Roma S, Choi YD, Matunis MJ et al (1988) Immunopurification of heterogeneous nuclear ribonucleoprotein particles reveals an assortment of RNA-binding proteins. Genes Dev 2(2):215–227. doi:10.1101/gad.2.2.215

    Article  PubMed  CAS  Google Scholar 

  2. Hay DC, Kemp GD, Dargemont C et al (2001) Interaction between hnRNPA1 and IkappaBalpha is required for maximal activation of NF-kappaB-dependent transcription. Mol Cell Biol 21(10):3482–3490. doi:10.1128/MCB.21.10.3482-3490.2001

    Article  PubMed  CAS  Google Scholar 

  3. Dreyfuss G, Matunis MJ, Pinol-Roma S et al (1993) hnRNP proteins and the biogenesis of mRNA. Annu Rev Biochem 62:289–321. doi:10.1146/annurev.bi.62.070193.001445

    Article  PubMed  CAS  Google Scholar 

  4. Iwanaga K, Sueoka N, Sato A et al (2005) Heterogeneous nuclear ribonucleoprotein B1 protein impairs DNA repair mediated through the inhibition of DNA-dependent protein kinase activity. Biochem Biophys Res Commun 333(3):888–895. doi:10.1016/j.bbrc.2005.05.180

    Article  PubMed  CAS  Google Scholar 

  5. Singh OP (2001) Functional diversity of hnRNP proteins. Indian J Biochem Biophys 38(3):129–134

    PubMed  CAS  Google Scholar 

  6. Krecic AM, Swanson MS (1999) hnRNP complexes: composition, structure, and function. Curr Opin Cell Biol 11(3):363–371. doi:10.1016/S0955-0674(99)80051-9

    Article  PubMed  CAS  Google Scholar 

  7. Liu J, Beqaj S, Yang Y et al (2001) Heterogeneous nuclear ribonucleoprotein-H plays a suppressive role in visceral myogenesis. Mech Dev 104(1–2):79–87. doi:10.1016/S0925-4773(01)00377-X

    Article  PubMed  CAS  Google Scholar 

  8. Chou MY, Rooke N, Turck CW et al (1999) hnRNP H is a component of a splicing enhancer complex that activates a c-src alternative exon in neuronal cells. Mol Cell Biol 19(1):69–77

    PubMed  CAS  Google Scholar 

  9. Rajagopalan LE, Westmark CJ, Jarzembowski JA et al (1998) hnRNP C increases amyloid precursor protein (APP) production by stabilizing APP mRNA. Nucleic Acids Res 26(14):3418–3423. doi:10.1093/nar/26.14.3418

    Article  PubMed  CAS  Google Scholar 

  10. Hassfeld W, Chan EK, Mathison DA et al (1998) Molecular definition of heterogeneous nuclear ribonucleoprotein R (hnRNP R) using autoimmune antibody: immunological relationship with hnRNP P. Nucleic Acids Res 26(2):439–445. doi:10.1093/nar/26.2.439

    Article  PubMed  CAS  Google Scholar 

  11. Rossoll W, Kroning AK, Ohndorf UM et al (2002) Specific interaction of Smn, the spinal muscular atrophy determining gene product, with hnRNP-R and gry-rbp/hnRNP-Q: a role for Smn in RNA processing in motor axons? Hum Mol Genet 11(1):93–105. doi:10.1093/hmg/11.1.93

    Article  PubMed  CAS  Google Scholar 

  12. Mourelatos Z, Abel L, Yong J et al (2001) SMN interacts with a novel family of hnRNP and spliceosomal proteins. EMBO J 20(19):5443–5452. doi:10.1093/emboj/20.19.5443

    Article  PubMed  CAS  Google Scholar 

  13. Huang J, Chen XH, Wu K et al (2005) Cloning and expression of a novel isoform of heterogeneous nuclear ribonucleoprotein-R. NeuroReport 16(7):727–730. doi:10.1097/00001756-200505120-00014

    Article  PubMed  CAS  Google Scholar 

  14. Huang J, Li SJ, Chen XH et al (2008) hnRNP-R regulates the PMA-induced c-fos expression in retinal cells. Cell Mol Biol Lett 13(2):303–311. doi:10.2478/s11658-008-0002-0

    Article  PubMed  CAS  Google Scholar 

  15. Peng ZY, Lee SC, Chen XH (2007) The expression and distribution of neural salient serine/arginine-rich protein 1 in rat retina. NeuroReport 18(16):1641–1644

    Article  PubMed  CAS  Google Scholar 

  16. Ghosh KK, Bujan S, Haverkamp S et al (2004) Types of bipolar cells in the mouse retina. J Comp Neurol 469(1):70–82. doi:10.1002/cne.10985

    Article  PubMed  Google Scholar 

  17. Carson JH, Barbarese E (2005) Systems analysis of RNA trafficking in neural cells. Biol Cell 97(1):51–62. doi:10.1042/BC20040083

    Article  PubMed  CAS  Google Scholar 

  18. Du Q, Melnikova IN, Gardner PD (1998) Differential effects of heterogeneous nuclear ribonucleoprotein K on Sp1- and Sp3-mediated transcriptional activation of a neuronal nicotinic acetylcholine receptor promoter. J Biol Chem 273(31):19877–19883. doi:10.1074/jbc.273.31.19877

    Article  PubMed  CAS  Google Scholar 

  19. Yano M, Okano HJ, Okano H (2005) Involvement of Hu and heterogeneous nuclear ribonucleoprotein K in neuronal differentiation through p21 mRNA post-transcriptional regulation. J Biol Chem 280(13):12690–12699. doi:10.1074/jbc.M411119200

    Article  PubMed  CAS  Google Scholar 

  20. Hua Y, Vickers TA, Okunola HL et al (2008) Antisense masking of an hnRNP A1/A2 intronic splicing silencer corrects SMN2 splicing in transgenic mice. Am J Hum Genet 82(4):834–848. doi:10.1016/j.ajhg.2008.01.014

    Article  PubMed  CAS  Google Scholar 

  21. Blanchette AR, Fuentes Medel YF, Gardner PD (2006) Cell-type-specific and developmental regulation of heterogeneous nuclear ribonucleoprotein K mRNA in the rat nervous system. Gene Expr Patterns 6(6):596–606. doi:10.1016/j.modgep.2005.11.008

    Article  PubMed  CAS  Google Scholar 

  22. Yang XL (2004) Characterization of receptors for glutamate and GABA in retinal neurons. Prog Neurobiol 73(2):127–150. doi:10.1016/j.pneurobio.2004.04.002

    Article  PubMed  CAS  Google Scholar 

  23. Kovacs KJ (1998) c-Fos as a transcription factor: a stressful (re)view from a functional map. Neurochem Int 33(4):287–297. doi:10.1016/S0197-0186(98)00023-0

    Article  PubMed  CAS  Google Scholar 

  24. Rossoll W, Jablonka S, Andreassi C et al (2003) Smn, the spinal muscular atrophy-determining gene product, modulates axon growth and localization of beta-actin mRNA in growth cones of motoneurons. J Cell Biol 163(4):801–812. doi:10.1083/jcb.200304128

    Article  PubMed  CAS  Google Scholar 

  25. Sommer B, Keinanen K, Verdoorn TA et al (1990) Flip and flop: a cell-specific functional switch in glutamate-operated channels of the CNS. Science 249(4976):1580–1585. doi:10.1126/science.1699275

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by grants from National Natural Science Foundation of China (30770661), Shanghai Leading Academic Discipline Project (B111), Shanghai Key project (07DJ14005), Shanghai Health Department (2006003) and Research Fund for the Doctoral Program of Higher Education of China (20070246053).

Author information

Authors and Affiliations

  1. Laboratory of Genomic Physiology, School of Life Sciences and State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai, 200032, China

    Zheng-Yu Peng, Jia Huang, Yong-Liang Shi, Xian-Hua Chen & Ping Xu

  2. Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China

    Shu-Chen Lee

Authors
  1. Zheng-Yu Peng
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jia Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shu-Chen Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yong-Liang Shi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xian-Hua Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ping Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Xian-Hua Chen or Ping Xu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Peng, ZY., Huang, J., Lee, SC. et al. The Expression Pattern of Heterogeneous Nuclear Ribonucleoprotein R in Rat Retina. Neurochem Res 34, 1083–1088 (2009). https://doi.org/10.1007/s11064-008-9878-3

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11064-008-9878-3

Keywords

Search

Navigation