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Early Anti-Oxidative and Anti-Proliferative Curcumin Effects on Neuroglioma Cells Suggest Therapeutic Targets

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Abstract

Curcumin (diferuloyl), from the Indian spice turmeric, reduces oxidative damage and induces apoptosis. Utilizing DNA microarrays, we have demonstrated that a low (5 μM) dose of curcumin added to a mixture of astrocytes and oligodendrocytes (C6 rat glioma cells) in culture for 24 and 48 h significantly modulates gene expression in four primary pathways: oxidative stress, cell cycle control, and DNA transcription and metabolism. Contribution of the pentose phosphate pathway to the pool of NADH upregulates glutathione and activates aldehyde oxidase. We have identified also several new genes, up- or downregulated by curcumin, namely, aldo-keto reductase, glucose-6-phosphate dehydrogenase, and aldehyde oxidase that protect against oxidative stress. The identification of several new cell cycle control genes, including the apoptosis-related protein (pirin) and insulin-like growth factor (IGF), and of the neurofilament M protein involved in neurogenesis suggests that curcumin may have applicability in the treatment of a spectrum of neurodegenerative diseases.

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Acknowledgment

This study was supported by NIH-AG19145 grant.

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Authors and Affiliations

  1. Department of Molecular and Cell Biology, University of California at Berkeley, 16 Barker Hall (102 Donner Lab), Berkeley, CA, 94720-3206, USA

    Heena D. Panchal, Chun Y. Lee, Jacqueline Ho & Paola S. Timiras

  2. Functional Genomics Laboratory, University of California at Berkeley, Berkeley, CA, 94720, USA

    Karen Vranizan & John Ngai

  3. Department of Molecular and Cell Biology, University of California, Berkeley, 269 LSA, Berkeley, CA, 94720-3200, USA

    John Ngai

Authors
  1. Heena D. Panchal
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  2. Karen Vranizan
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  3. Chun Y. Lee
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  4. Jacqueline Ho
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  6. Paola S. Timiras
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Correspondence to Paola S. Timiras.

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Panchal, H.D., Vranizan, K., Lee, C.Y. et al. Early Anti-Oxidative and Anti-Proliferative Curcumin Effects on Neuroglioma Cells Suggest Therapeutic Targets. Neurochem Res 33, 1701–1710 (2008). https://doi.org/10.1007/s11064-008-9608-x

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  • DOI: https://doi.org/10.1007/s11064-008-9608-x

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