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Influence of Integrin-blocking Peptide on Gadolinium- and Hypertonic Shrinking-induced Neurotransmitter Release in Rat Brain Synaptosomes

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Abstract

Polyvalent cations and hypertonic shrinking of presynaptic endings lead to calcium-independent exocytosis in various synapses. In the present study we have investigated the contribution of integrins to this phenomenon. It was found that hypertonic shrinking, polyvalent cations ruthenium red and gadolinium results in dose-dependent calcium-independent neurotransmitter release in rat brain synaptosomes. The exocytotic mechanism of neurotransmitter release induced by 300 μM gadolinium was additionally verified by the fluorescent dye FM2-10. We found that 200 μM of RGDS peptide, an inhibitor of integrins, decreased polyvalent gadolinium-induced [3H]d-aspartate release by 26%. This compound had no effect upon hypertonicity-induced release. The peptide RGES, a negative control for RGDS; genistein, an inhibitor of tyrosine kinases; and citrate, an inhibitor of lanthanides-induced aggregation were ineffective in both cases. Therefore, we have shown that integrins did not influence hypertonicity-evoked [3H]d-aspartate release, but partially mediated that evoked by gadolinium ions.

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Acknowledgments

This work was supported by The Physiological Society “Centres of excellence” scheme. Initial experiments were supported by The Wellcome Trust (grant 069417/Z/02/Z). We thank Dr. Ewan Smith for improvement of English. We thank Prof. E. Slobozhanina for access to aggregometer and Dr. N. Paulava for assistance in measurement of synaptosomes aggregation.

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  1. Liudmila P. Lapatsina

    Present address: Max-Delbrück-Centrum für Moleculare Medizin, Berlin, Germany

Authors and Affiliations

  1. Institute of Biophysics and Cell Engineering, Akademicheskaya St., 27, Minsk, 220072, Belarus

    Tatyana V. Waseem, Liudmila P. Lapatsina & Sergei V. Fedorovich

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  1. Tatyana V. Waseem
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  2. Liudmila P. Lapatsina
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  3. Sergei V. Fedorovich
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Correspondence to Sergei V. Fedorovich.

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Waseem, T.V., Lapatsina, L.P. & Fedorovich, S.V. Influence of Integrin-blocking Peptide on Gadolinium- and Hypertonic Shrinking-induced Neurotransmitter Release in Rat Brain Synaptosomes. Neurochem Res 33, 1316–1324 (2008). https://doi.org/10.1007/s11064-007-9585-5

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  • DOI: https://doi.org/10.1007/s11064-007-9585-5

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