Abstract
Neuronal nitric oxide synthase (nNOS) has been implicated to influence peripheral nerve lesion and regeneration. Post-synaptic density-95 (PSD-95) is one of nNOS-anchoring proteins and plays an important role in specifying the sites of reaction of NO in nervous system. Here we established a rat sciatic nerve crush (SNC) model to examine the spatiotemporal expression of PSD-95 and nNOS. At gene levels, PSD-95 mRNA diminished shortly after crush, and significantly elevated from 2 days to 2 weeks, whereas nNOS decreased progressively post-operation, reached the valley at 1 day, and markedly up-regulated from 1 to 2 weeks after SNC. The expression of both molecules returned to the control level at 4 weeks post-injury. At protein levels, PSD-95 and nNOS underwent the similar changes as their gene expression except for a time lag during up-regulating. At their peak expression, PSD-95 co-labeled with nNOS in Schwann cells (SCs) of sciatic nerve within 0.5 mm from the lesion site, but had few colocalization in axons. In addition, the interaction between PSD-95 and nNOS enhanced significantly at 2 weeks after SNC. These results suggest a correlation of PSD-95 up-regulation with nNOS in reactive SCs of crushed sciatic nerve, which may lead to understanding the function of PSD-95 during peripheral nerve regeneration.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (No.30300099 and NO.30770488) and Natural Science Foundation of Jiangsu province (No.BK2003035 and No.BK2006547) and “Liu Da Ren Cai Gao Feng” Financial Assistant Project of Jiangsu Province (NO.2).
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Shangfeng Gao and Min Fei contributed equally to this work.
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Gao, S., Fei, M., Cheng, C. et al. Spatiotemporal Expression of PSD-95 and nNOS After Rat Sciatic Nerve Injury. Neurochem Res 33, 1090–1100 (2008). https://doi.org/10.1007/s11064-007-9555-y
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DOI: https://doi.org/10.1007/s11064-007-9555-y