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Effects of Chronic Ethanol Consumption on Brain Synaptosomes and Protective Role of Betaine

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Abstract

To evaluate the cytotoxic effects of chronic ethanol consumption on brain cerebral synaptosomes and preventive role of betaine as a methyl donor andS-adenosylmethionine precursor, 24 male Wistar rats were divided into three groups: control, ethanol (8 g/kg/day) and ethanol plus betaine(0.5% w/v) group. Animals were fed 60 ml/diet per day for two months, then sacrificed. Malondialdehyde (MDA), protein carbonyl contents and adenosine deaminase (ADA) activities were determined in synaptosomal/mitochondrial enriched fraction isolated from rat cerebral cortexes. When compared to controls, ethanol containing diet significantly increased MDA levels (P < 0.05), also increased protein carbonyl levels and adenosine deaminase activities. But these were not statistically significant (P > 0.05). However, adding betaine to ethanol containing diet caused a significant decrease in MDA, protein carbonyl levels and adenosine deaminase activities (P < 0.05). These results indicate that betaine may appear as a protective nutritional agent against cytotoxic brain damage induced by chronic ethanol consumption.

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Authors and Affiliations

  1. Department of Biochemistry, School of Medicine, Eskisehir Osmangazi University, Eskisehir, 26480, Turkey

    Gungor Kanbak, Okan Can Arslan, Ali Dokumacioglu, Kazim Kartkaya & Mine Erden İnal

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  1. Gungor Kanbak
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  2. Okan Can Arslan
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  3. Ali Dokumacioglu
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Correspondence to Okan Can Arslan.

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Kanbak, G., Arslan, O.C., Dokumacioglu, A. et al. Effects of Chronic Ethanol Consumption on Brain Synaptosomes and Protective Role of Betaine. Neurochem Res 33, 539–544 (2008). https://doi.org/10.1007/s11064-007-9472-0

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  • DOI: https://doi.org/10.1007/s11064-007-9472-0

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