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Transplantation of Foetal Neural Stem Cells into the Rat Hippocampus During Trimethyltin-Induced Neurodegeneration

  • Special Issue In Honor of Naren Banik
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Abstract

The present study investigates the survival and fate of neural stem cells/progenitor cells (NSC/NPCs) homografted into the hippocampus of rats treated with trimethyltin (TMT), a potent neurotoxicant considered a useful tool to obtain a well characterized model of neurodegeneration, to evaluate their possible role in the reparative mechanisms that accompany neurodegenerative events. NSC/NPCs expressing eGFP by lentivirus-mediated infection were stereotaxically grafted into the hippocampus of TMT-treated animals and controls. Two weeks after transplantation surviving NSC/NPCs were detectable in 60% of TMT-treated animals and 30% of controls, while 30 days after transplantation only 40% of TMT-treated animals showed surviving grafted cells, which were undetectable in controls. At both times investigated, while grafted NSC/NPCs differentiated into neurons or astrocytes could be observed in addition to undifferentiated NSC/NPCs, we did not find evidence of structural integration of grafted cells into the main site of hippocampal lesion leading to appreciable repair.

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Acknowledgments

This work was partially supported by The Nando Peretti Foundation, ATENA Onlus, by funds from Università Cattolica del S.Cuore to M.C.G., S.G and F.M., and from Ministero della Salute and Ministero dell’Università to F.M. We would also like to thank Dr. Marco Molinari, Fulvio Florenzano and Maria Teresa Viscomi for their assistance and technical support in confocal microscope use.

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Authors and Affiliations

  1. Institute of Anatomy and Cell Biology, Università Cattolica del Sacro Cuore, Largo F. Vito 1, 00168, Rome, Italy

    Maria Concetta Geloso, Stefano Giannetti & Fabrizio Michetti

  2. Institute of Neurosurgery, Università Cattolica del Sacro Cuore, Rome, Italy

    Carlo Cenciarelli, Manuela Budoni & Giulio Maira

  3. Institute of Neurobiology and Molecular Medicine, CNR, Rome, Italy

    Patrizia Casalbore

Authors
  1. Maria Concetta Geloso
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  2. Stefano Giannetti
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  3. Carlo Cenciarelli
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  4. Manuela Budoni
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  5. Patrizia Casalbore
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  6. Giulio Maira
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  7. Fabrizio Michetti
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Corresponding author

Correspondence to Fabrizio Michetti.

Additional information

Maria Concetta Geloso and Stefano Giannetti contributed equally to this work.

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Geloso, M.C., Giannetti, S., Cenciarelli, C. et al. Transplantation of Foetal Neural Stem Cells into the Rat Hippocampus During Trimethyltin-Induced Neurodegeneration. Neurochem Res 32, 2054–2061 (2007). https://doi.org/10.1007/s11064-007-9353-6

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  • DOI: https://doi.org/10.1007/s11064-007-9353-6

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