Abstract
We earlier reported that overexpression of glia maturation factor (GMF) in cultured astrocytes enhances the production of brain-derived neurotrophic factor (BDNF). The current study was conducted to find out whether BDNF production is impaired in animals devoid of GMF. To this end GMF-knockout (KO) mice were subjected to exercise and the neurotrophin mRNAs were determined by real-time RT-PCR. Compared to wild-type (WT) mice, there is a decrease in exercise-induced BDNF in the KO mice. The observation was correlated with the finding that, in WT mice, exercise increases GMF expression. The results are consistent with the hypothesis that GMF is necessary for exercise-induction of BDNF, and that GMF may promote neuroprotection through BDNF production.
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Abbreviations
- BDNF:
-
brain-derived neurotrophic factor
- CREB:
-
cAMP response element binding protein
- GM-CSF:
-
granulocyte-macrophage-colony stimulating factor
- GMF:
-
glia maturation factor
- KO:
-
knockout
- MAP kinase:
-
mitogen-activated protein kinase
- NF-κB:
-
nuclear factor-κB
- NGF:
-
nerve growth factor
- NT3:
-
neurotrophin 3
- RT-PCR:
-
reverse transcription-polymerase chain reaction
- WT:
-
wild type
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Acknowledgements
This work was supported by the Department of Veterans Affairs Merit Review Award (to A. Z. and R. L.) and by NIH grant NS-47145 (to A.Z.). We thank Timothy J., Brennan for the use of the treadmill machine and Mariam Bridget Zimmerman for statistical analysis. GMF-knockout mice were produced at the University of Iowa Gene Targeting Core Facility.
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Zaheer, A., Haas, J.T., Reyes, C. et al. GMF-Knockout Mice are Unable to Induce Brain-Derived Neurotrophic Factor after Exercise. Neurochem Res 31, 579–584 (2006). https://doi.org/10.1007/s11064-006-9049-3
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DOI: https://doi.org/10.1007/s11064-006-9049-3