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Dual Effect of Pyruvate in Isolated Nerve Terminals: Generation of Reactive Oxygen Species and Protection of Aconitase

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Abstract

Generation of reactive oxygen species (ROS) in synaptosomes was investigated in the presence of different substrates. When pyruvate was used as a substrate an increased rate of hydrogen peroxide formation was detected by the Amplex Red fluorescent assay, but aconitase, which is known to be a highly sensitive enzyme to ROS was not inhibited. In contrast, pyruvate exerted a partial protection on aconitase against a time-dependent inactivation that occurred when synaptosomes were incubated in the absence of substrates. Disruption of synaptosomal membranes with Triton X-100 prevented the protective effect of pyruvate. It is suggested that citrate and/or isocitrate formed in the metabolism of pyruvate could be responsible for a partial protection of aconitase. Therefore while pyruvate could have a prooxidant effect it could also exert a protective effect on the aconitase.

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  1. Department of Medical Biochemistry, Semmelweis University and Neurobiochemical Group, Hungarian Academy of Sciences, Szentágothai János Knowledge Center, Budapest, Hungary

    Laszlo Tretter, Balint Liktor & Vera Adam-Vizi

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  1. Laszlo Tretter
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  2. Balint Liktor
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  3. Vera Adam-Vizi
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Correspondence to Vera Adam-Vizi.

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Special issue dedicated to Dr. Bernd Hamprecht.

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Tretter, L., Liktor, B. & Adam-Vizi, V. Dual Effect of Pyruvate in Isolated Nerve Terminals: Generation of Reactive Oxygen Species and Protection of Aconitase. Neurochem Res 30, 1331–1338 (2005). https://doi.org/10.1007/s11064-005-8805-0

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