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Hypothalamic Proline-Rich Polypeptide is an Oxidative Burst Regulator

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Abstract

The AGAPEPAEPAQPGVY proline-rich polypeptide (PRP) was isolated from neurosecretory granules of the bovine neurohypophysis; it is produced by N. supraopticus and N. paraventricularis. PRP possesses immune-modulating activity, preventing the death of Gram-negative bacteria-infected mice. Here we show that PRP does not affect human peripheral blood neutrophlis and monocytes phagocytosis but dramatically enhances spontaneous or fMLP- and PMA-induced, and also phagocytosis-dependent, oxidative burst. We demonstrated the regulatory role of PRP on the oxidative burst induction of normal and relapsing inflammatory disease (Behcet’s disease and familial Mediterranean fever) neutrophils and monocytes. Our results suggest a previously undescribed role for the hypothalamic peptide within primary activated neutrophils and monocytes, since we provide evidence that PRP can differentially regulate both chemotaxis- and phagocytosis-dependent oxidative burst in normal and inflammatory disease effector cells.

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Authors and Affiliations

  1. Department of Neurohormone Biochemistry, H. Buniatian Institute of Biochemistry, NAS RA, 5/1 Sevag Street, Yerevan, 375014, Republic of Armenia

    Tigran K. Davtyan, Hayk M. Manukyan & Armen A. Galoyan

  2. Laboratory of Immunology and Virology, Republic of Armenia

    Tigran K. Davtyan

  3. Department of Rheumatology, National Institute of Health, Republic of Armenia

    Nana R. Mkrtchyan

  4. Department of Pathophysiology, Yerevan State Medical University, Republic of Armenia

    Samvel A. Avetisyan

  5. Department of Internal Medicine, Yerevan State Medical University, Republic of Armenia

    Gagik S. Hakopyan

Authors
  1. Tigran K. Davtyan
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  2. Hayk M. Manukyan
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  3. Gagik S. Hakopyan
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  4. Nana R. Mkrtchyan
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  5. Samvel A. Avetisyan
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  6. Armen A. Galoyan
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Correspondence to Tigran K. Davtyan.

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Davtyan, T.K., Manukyan, H.M., Hakopyan, G.S. et al. Hypothalamic Proline-Rich Polypeptide is an Oxidative Burst Regulator. Neurochem Res 30, 297–309 (2005). https://doi.org/10.1007/s11064-005-2603-6

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  • DOI: https://doi.org/10.1007/s11064-005-2603-6

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