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Accumulation of Acrolein–Protein Adducts after Traumatic Spinal Cord Injury

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Abstract

Reactive oxygen species and resultant lipid peroxidation (LPO) have been associated with central nervous system trauma. Acrolein (2-propenal) and 4-hydroxynonenal (HNE) are the most toxic byproducts of LPO, with detrimental effects in various types of cells. In this study, we used immunoblotting techniques to detect the accumulation of protein-bound acrolein and HNE. We report that protein-bound acrolein and HNE were significantly increased in guinea pig spinal cord following a controlled compression injury. The acrolein and HNE protein-adducts increased in the damaged spinal cord as early as 4 h after injury, reached a peak at 24 h after injury, and remained at a significantly high level up to 7 days after injury. Such increase of protein adducts was also observed in the adjacent segments of the injury site beginning at 24 h post injury. These results suggest that products of lipid peroxidation, especially acrolein, may play a critical role in the secondary neuronal degeneration, which follows mechanical insults.

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References

  1. E. D. Hall (1993) ArticleTitleLipid antioxidants in acute central nervous system injury Ann. Emerg. Med 22 1022–1007 Occurrence Handle1:STN:280:ByyB2snpslU%3D Occurrence Handle8503522

    CAS  PubMed  Google Scholar 

  2. A. Lewen P. Matz P. H. Chan (2000) ArticleTitleFree radical pathways in CNS injury J Neurotrauma 17 871–890 Occurrence Handle1:STN:280:DC%2BD3crgvFOjtA%3D%3D Occurrence Handle11063054

    CAS  PubMed  Google Scholar 

  3. J. T. Povlishock H. A. Kontos (1992) ArticleTitleThe role of oxygen radicals in the pathobiology of traumatic brain injury Human Cell 5 345–353 Occurrence Handle1:STN:280:ByyB1czjt1I%3D Occurrence Handle1304799

    CAS  PubMed  Google Scholar 

  4. H. Esterbauer R. J. Schaur H. Zollner (1991) ArticleTitleChemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes Free Rad. Biol. Med 11 81–128 Occurrence Handle1:CAS:528:DyaK3MXmsFSnsbo%3D Occurrence Handle1937131

    CAS  PubMed  Google Scholar 

  5. J. P. Kehrer S. S. Biswal (2000) ArticleTitleThe molecular effects of acrolein Toxicol. Sci 57 6–15 Occurrence Handle1:CAS:528:DC%2BD3cXmslyls7g%3D Occurrence Handle10966506

    CAS  PubMed  Google Scholar 

  6. K. Uchida (2003) ArticleTitle4-Hydroxy-2-nonenal: a product and mediator of oxidative stress Prog. Lipid Res 42 318–343 Occurrence Handle1:CAS:528:DC%2BD3sXis1yqtro%3D Occurrence Handle12689622

    CAS  PubMed  Google Scholar 

  7. J. Luo R. Shi (2004) ArticleTitleAcrolein induces axolemmal disruption, oxidative stress, and mitochondrial impairment in spinal cord tissue Neurochem. Int 44 475–486 Occurrence Handle1:CAS:528:DC%2BD2cXhtFWitbc%3D Occurrence Handle15209416

    CAS  PubMed  Google Scholar 

  8. S. A. Baldwin R. Broderick D. Osbourne G. Waeg D. A. Blades S. W. Scheff (1998) ArticleTitleThe presence of 4-hydroxynonenal/protein complex as an indicator of oxidative stress after experimental spinal cord contusion in a rat J. Neurosurg 88 874–883 Occurrence Handle1:CAS:528:DyaK1cXjtVShsr8%3D Occurrence Handle9576257

    CAS  PubMed  Google Scholar 

  9. J. E. Springer R. D. Azbill R. J. Mark J. G. Begley G. Waeg M. P. Mattson (1997) ArticleTitle4-hydroxynonenal, a lipid peroxidation product, rapidly accumulates following traumatic spinal cord injury and inhibits glutamate uptake J. Neurochem 68 2469–2476 Occurrence Handle1:STN:280:ByiA3cbjt1I%3D Occurrence Handle9166741

    CAS  PubMed  Google Scholar 

  10. R. Shi J. Luo M. A. Peasley (2002) ArticleTitleAcrolein inflicts axonal membrane disruption and conduction loss in isolated guinea pig spinal cord Neuroscience 115 337–340 Occurrence Handle1:CAS:528:DC%2BD38XotlymsbY%3D Occurrence Handle12421600

    CAS  PubMed  Google Scholar 

  11. M. A. Peasley R. Shi (2003) ArticleTitleIschemic insult exacerbates acrolein-induced conduction loss and axonal membrane disruption in guinea pig spinal cord white matter J. Neurol. Sci 216 23–32 Occurrence Handle1:CAS:528:DC%2BD3sXos1Kmu7c%3D Occurrence Handle14607299

    CAS  PubMed  Google Scholar 

  12. W. R. Markesbery M. A. Lovell (1998) ArticleTitleFour-hydroxynonenal, a product of lipid peroxidation, is increased in the brain in Alzheimer’s disease Neurobiol. Aging 19 33–36 Occurrence Handle1:CAS:528:DyaK1cXisFyrtLg%3D Occurrence Handle9562500

    CAS  PubMed  Google Scholar 

  13. Lovell, M. A., Xie, C. and Markesbery, W. R. 2001. Acrolein is increased in Alzheimer’s disease brain and is toxic to primary hippocampal cultures. Neurobiol. Aging 187–194.

  14. N. Y. Calingasan K. Uchida G. E. Gibson (1999) ArticleTitleProtein-bound acrolein: a novel marker of oxidative stress in Alzheimer’s disease J. Neurochem 72 751–756 Occurrence Handle1:CAS:528:DyaK1MXmt1ymug%3D%3D Occurrence Handle9930749

    CAS  PubMed  Google Scholar 

  15. K. Uchida M. Kanematsu K. Sakai T. Matsuda N. Hattori Y. Mizuno D. Suzuki T. Miyata N. Noguchi et al. (1998) ArticleTitleProtein-bound acrolein: potential markers for oxidative stress Proc. Nat. Aca. Sci. USA 95 4882–4887 Occurrence Handle1:CAS:528:DyaK1cXivVyqurc%3D

    CAS  Google Scholar 

  16. K. Uchida M. Kanematsu Y. Morimitsu T. Osawa N. Noguchi E Niki (1998) ArticleTitleAcrolein is a product of lipid peroxidation reaction. Formation of free acrolein and its conjugate with lysine residues in oxidized low density lipoproteins J. Biol. Chem 273 16058–16066 Occurrence Handle1:CAS:528:DyaK1cXkt1Ghtbs%3D Occurrence Handle9632657

    CAS  PubMed  Google Scholar 

  17. R. B. Borgens R. Shi (2000) ArticleTitleImmediate recovery from spinal cord injury through molecular repair of nerve membranes with polyethylene glycol FASEB J 14 27–35 Occurrence Handle1:CAS:528:DC%2BD3cXltFKgug%3D%3D Occurrence Handle10627277

    CAS  PubMed  Google Scholar 

  18. R. B. Borgens R. Shi D. Bohnert (2002) ArticleTitleBehavioral recovery from spinal cord injury following delayed application of polyethylene glycol J. Exp. Biol 205 1–12 Occurrence Handle11818407

    PubMed  Google Scholar 

  19. K. Uchida E. R. Stadtman (2000) ArticleTitleQuantitation of 4-hydroxynonenal protein adducts Methods Mol. Biol 99 25–34 Occurrence Handle1:CAS:528:DC%2BD3cXktlWltL0%3D Occurrence Handle10909074

    CAS  PubMed  Google Scholar 

  20. D. P. Ghilarducci R. S. Tjeerdema (1995) ArticleTitleFate and effects of acrolein Rev Environ. Contam. Toxicol 144 95–146 Occurrence Handle1:CAS:528:DyaK28XhsFWmsrc%3D

    CAS  Google Scholar 

  21. I. Fridovich (1978) ArticleTitleThe biology of oxygen radicals Science 201 875–880 Occurrence Handle1:CAS:528:DyaE1cXlvFart7g%3D Occurrence Handle210504

    CAS  PubMed  Google Scholar 

  22. R. Shi (2004) ArticleTitleThe danamics of axolemal disruption in guinea pig spinal cord following compression J. Neurocytology 33 203–211

    Google Scholar 

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Authors and Affiliations

  1. Department of Basic Medical Sciences, Institute for Applied Neurology, Center for Paralysis Research, Purdue University, West Lafayette, IN, 47907, USA

    Jian Luo, Koji Uchida & Riyi Shi

  2. Laboratory of Food and Biodynamics Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan

    Koji Uchida

Authors
  1. Jian Luo
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  2. Koji Uchida
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  3. Riyi Shi
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Correspondence to Riyi Shi.

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Luo, J., Uchida, K. & Shi, R. Accumulation of Acrolein–Protein Adducts after Traumatic Spinal Cord Injury. Neurochem Res 30, 291–295 (2005). https://doi.org/10.1007/s11064-005-2602-7

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  • DOI: https://doi.org/10.1007/s11064-005-2602-7

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