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Alzheimer’s Disease: Soluble Oligomeric Aβ(1–40) Peptide in Membrane Mimic Environment from Solution NMR and Circular Dichroism Studies

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Abstract

Amyloid beta peptide (Aβ) is a small peptide present in normal cells and aggregated Aβ is the main constituent of the extracellular amyloid plaques found in Alzheimer’s disease (AD) brain. Recent studies suggest that soluble Aβ oligomers are neurotoxic rather than amyloid fibrils found in amyloid plaques. This study using multidimensional NMR spectroscopy and circular dichroism (CD) provides the first direct evidence that alterations in membrane structure can trigger the conversion of soluble α-helical monomeric Aβ into oligomeric Aβ in a β-sheet conformation.

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Authors and Affiliations

  1. Neurophysics Laboratory, Department of Psychiatry, Western Psychiatric Institute and Clinic, University of Pittsburgh Medical Center, Pittsburgh, PA, USA

    Pravat K. Mandal & Jay W. Pettegrew

  2. Neurophysics Laboratory, Psychiatry, Neurology and Health Services Administration, University of Pittsburgh, A741 Crabtree Hall/GSPH, 130 DeSoto Street, Pittsburgh, PA, 15213, USA

    Jay W. Pettegrew

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  1. Pravat K. Mandal
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  2. Jay W. Pettegrew
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Correspondence to Jay W. Pettegrew.

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Mandal, P.K., Pettegrew, J.W. Alzheimer’s Disease: Soluble Oligomeric Aβ(1–40) Peptide in Membrane Mimic Environment from Solution NMR and Circular Dichroism Studies. Neurochem Res 29, 2267–2272 (2004). https://doi.org/10.1007/s11064-004-7035-1

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