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Histological Co-Localization of Iron in Aß Plaques of PS/APP Transgenic Mice

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This study confirms the presence of iron, co-localized with Aβ plaques, in PS/APP mouse brain, using Perls‘ stain for Fe3+ supplemented by 3,3′-diaminobenzidine (DAB) and Aβ immunohistochemistry in histological brains sections fixed with formalin or methacarn. In this study, the fixation process and the slice thickness did not interfere with the Perls’ technique. The presence of iron in ß-amyloid plaques in PS/APP transgenic mice, a model of Alzheimer’s disease (AD) pathology, may explain previous reports of reductions of transverse relaxation time (T2) in MRI studies and represent the source of the intrinsic Aß plaque MR contrast in this model.

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Authors and Affiliations

  1. Center for Advanced Brain Imaging, The Nathan Kline Institute, 140 Old Orangeburg Road, Orangeburg, NY, 10962, USA

    Maria F. Falangola, Sang-Pil Lee & Joseph A. Helpern

  2. Center for Dementia Research, The Nathan Kline Institute, 140 Old Orangeburg Road, Orangeburg, NY, 10962, USA

    Ralph A. Nixon & Karen Duff

  3. Department of Psychiatry, New York University School of Medicine, New York, NY, 10016, USA

    Ralph A. Nixon, Karen Duff & Joseph A. Helpern

  4. Department of Physiology and Neuroscience, New York University School of Medicine, New York, NY, 10016, USA

    Ralph A. Nixon & Joseph A. Helpern

  5. Department of Radiology, New York University School of Medicine, New York, NY, 10016, USA

    Maria F. Falangola & Joseph A. Helpern

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  1. Maria F. Falangola
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  2. Sang-Pil Lee
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  5. Joseph A. Helpern
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Correspondence to Maria F. Falangola.

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Falangola, M.F., Lee, SP., Nixon, R.A. et al. Histological Co-Localization of Iron in Aß Plaques of PS/APP Transgenic Mice. Neurochem Res 30, 201–205 (2005). https://doi.org/10.1007/s11064-004-2442-x

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  • DOI: https://doi.org/10.1007/s11064-004-2442-x

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