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Prognostic value of NUSAP1 in progression and expansion of glioblastoma multiforme

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Abstract

Nucleolar and spindle-associated protein (NUSAP1) is a microtubule and chromatin-binding protein that stabilizes microtubules to prevent depolymerization, maintains spindle integrity. NUSAP1 could cross-link spindles into aster-like structures, networks and fibers. It has also been found to play roles in progression of several cancers. However, the potential correlation between NUSAP1 and clinical outcome in patients with glioblastoma multiforme (GBM) remains largely unknown. In the current study, we demonstrated that NUSAP1 was significantly up-regulated in GBM tissues compared with adult non-tumor brain tissues both in a validated cohort and a TCGA cohort. In addition, Kaplan–Meier analysis indicated that patients with high NUSAP1 expression had significantly lower OS (P = 0.0027). Additionally, in the TCGA cohort, NUSAP1 expression was relatively lower in GBM patients within the neural and mesenchymal subtypes compared to other subtypes, and associated with the status of several genetic aberrations such as PTEN deletion and wild type IDH1. The present study provides new insights and evidence that NUSAP1 over-expression was significantly correlated with progression and prognosis of GBM. Furthermore, knockdown of NUSAP1 revealed its regulation on G2/M progression and cell proliferation (both in vitro and in vivo). These data demonstrate that NUSAP1 could serve as a novel prognostic biomarker and a potential therapeutic target for GBM.

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Abbreviations

GBM:

Glioblastoma multiforme

NUSAP1:

Nucleolar-spindle associated protein

TCGA:

The Cancer Genome Atlas

CNS:

Central nervous system

SPF:

Specific pathogen-free

shRNA:

Short hairpin RNA

qRT-PCR:

Quantitative real-time polymerase chain reaction

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Acknowledgements

This study was supported by National Natural Science Foundation of China (81472739; 81602183; 81702457), Natural Science Foundation of Jiangsu province, China (BK20151214).

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Author notes

  1. Zhiyuan Qian, Yuping Li and Jiawei Ma have contributed equally to this work.

Authors and Affiliations

  1. Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Rd, Suzhou, 215004, China

    Zhiyuan Qian, Jiawei Ma, Yanping Xue, Yujun Xi, Lei Hong, Xiaoyan Ji, Yanming Chen, Minfeng Sheng, Yujing Sheng, Lin Yang, Jiachi Liu, Xingliang Dai, Jia Shi, Tao Xie & Jun Dong

  2. Department of Neurosurgery, Clinical Medical College of Yangzhou University, 98 Nantong West-Road, Yangzhou, 225001, China

    Yuping Li

  3. Department of Pathology, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Rd, Suzhou, 215004, China

    Xiaoxiao Dai & Yongsheng Zhang

  4. Brain Tumor Lab, Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, Soochow University, 1055 Sanxiang Rd, Suzhou, 215004, China

    Yujun Xi & Jun Dong

Authors
  1. Zhiyuan Qian
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Correspondence to Jun Dong.

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Qian, Z., Li, Y., Ma, J. et al. Prognostic value of NUSAP1 in progression and expansion of glioblastoma multiforme. J Neurooncol 140, 199–208 (2018). https://doi.org/10.1007/s11060-018-2942-1

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  • DOI: https://doi.org/10.1007/s11060-018-2942-1

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