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miR-335 promotes cell proliferation by directly targeting Rb1 in meningiomas

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Abstract

Meningiomas, one of the most common benign brain tumors in humans, arise from arachnoid cells in the brain meninges. Our investigations have revealed that miR-335 is a typical microRNA overexpressed in meningiomas in humans. Characterization of the effects of miR-335 overexpression in meningiomas demonstrated that elevated levels of miR-335 increased cell growth and inhibited cell cycle arrest in the G0/G1 phase in vitro; in addition, reduction of the miR-335 levels had the opposite effect on tumor growth and progression. Further, previous studies have shown that the mechanism of effect of miR-335 on the proliferation of meningioma cells is associated with alterations in the expression of human retinoblastoma 1 (Rb1). Our results indicate that miR-335 plays an essential role in the proliferation of meningioma cells by directly targeting the Rb1 signaling pathway. Thus, our results highlight a novel molecular interaction between miR-335 and Rb1, and miR-335 may represent a potential novel therapeutic agent to target the proliferation of meningioma cells.

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Acknowledgments

This work was supported by the China Natural Science Foundation (81072078, 81000963, 30200335 and 30872657), Jiangsu Province’s 333 Talent Project Foundation (BRA2011046), the Kunshan Social Development Foundation (Grant Number: KS1006, KS1009), and the Suzhou Social Development Foundation (SYS201063).

Conflict of interest

All authors have declared the sources of research funding for this manuscript and have no financial or other contractual agreements that might cause (or be perceived as causes of) conflicts of interest.

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Authors and Affiliations

  1. Department of Neurosurgery, The First People’s Hospital of Kunshan Affiliated with Jiangsu University, Suzhou, 215300, People’s Republic of China

    Lei Shi, Shuguang Zhang & Tianhong Pan

  2. Department of Neurosurgery, Suzhou Kowloon Hospital Affiliated with Shanghai Jiao Tong University School of Medicine, Suzhou, 215021, People’s Republic of China

    Dongyi Jiang, Yi Wan & Zhimin Wang

  3. Department of Neurosurgery, Fourth Affiliated Yancheng Hospital of Nantong University, Yancheng, 224000, People’s Republic of China

    Guan Sun

  4. Biomechanics and Medical Information Institute, Beijing University of Technology, Beijing, 100022, People’s Republic of China

    Yanjun Zeng

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  1. Lei Shi
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  2. Dongyi Jiang
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  3. Guan Sun
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  4. Yi Wan
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  5. Shuguang Zhang
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  6. Yanjun Zeng
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  7. Tianhong Pan
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  8. Zhimin Wang
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Corresponding authors

Correspondence to Yanjun Zeng, Tianhong Pan or Zhimin Wang.

Additional information

Lei Shi, Dongyi Jiang, Guan Sun and Yi Wan have contributed equally to this study.

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Shi, L., Jiang, D., Sun, G. et al. miR-335 promotes cell proliferation by directly targeting Rb1 in meningiomas. J Neurooncol 110, 155–162 (2012). https://doi.org/10.1007/s11060-012-0951-z

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  • DOI: https://doi.org/10.1007/s11060-012-0951-z

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