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Expression of RECK in endothelial cells of glioma: comparison with CD34 and VEGF expressions

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Abstract

Angiogenesis is thought to be involved in the progression of glioma grades, and reversion-inducing cysteine-rich protein with Kazal motifs (RECK) has been said to be involved in maturation of vessels. In this study, we aimed to determine whether high micro-vessel density (MVD) expressed by RECK in glioma tissue is correlated with grades of glioma. We also compared RECK expression with that of the formerly known vessel marker, CD34, and vascular endothelial growth factor (VEGF). RECK, CD34, and VEGF immuno-reactivities of 72 glioma tissues were studied. RECK was seen in microvessels of glioma tissues. CD34 showed a similar pattern to RECK, whereas VEGF showed positive staining in cytoplasm of tumor cells and endothelial cells. Average MVD with RECK was 107.6 microvessels (range 7–290). RECK was positively correlated with grades of glioma. RECK and CD34 also showed a strong correlation (P = 0.001). Higher frequency of VEGF staining was also correlated with higher grade of glioma. This is the first study describing expression of RECK in glioma, and its angiogenesis-related nature may provide a potential therapeutic target for glioma treatment in the future.

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Acknowledgments

The authors are very grateful to Ms. Tomoko Nishizawa and Dr. Takashi Ehara from the department of pathology, Shinshu University School of Medicine, for their help with the immunohistochemistry staining technique. This study was supported in part by the Research Fund for Health Care Promotion Plan in Josho Area, Nagano Prefecture, from the Ministry of Health, Labour and Welfare of Japan, and the Grants-in-Aid for the Foreign Researcher Program of Shinshu Public Utility Foundation for Promotion of Medical Sciences.

Conflict of interest

The authors have no financial, commercial, legal, or professional relationship with other organizations or with the people working with us that may exert an influence on this research. Therefore, we have no conflict of interest in the making of this paper.

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Authors and Affiliations

  1. Department of Neurosurgery, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto, 390-8621, Japan

    Nunung Nur Rahmah, Keiichi Sakai & Kazuhiro Hongo

  2. Department of Neurosurgery, Shinshu Ueda Medical Center, Ueda, Japan

    Keiichi Sakai

  3. Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan

    Kenji Sano

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  1. Nunung Nur Rahmah
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  2. Keiichi Sakai
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  4. Kazuhiro Hongo
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Correspondence to Keiichi Sakai.

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Rahmah, N.N., Sakai, K., Sano, K. et al. Expression of RECK in endothelial cells of glioma: comparison with CD34 and VEGF expressions. J Neurooncol 107, 559–564 (2012). https://doi.org/10.1007/s11060-011-0778-z

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  • DOI: https://doi.org/10.1007/s11060-011-0778-z

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